Literature DB >> 9708362

Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides.

C L Smith1, A C Simmonds, A L Hamilton, D L Martin, A G Lashford, D Loakes, F Hill, D M Brown.   

Abstract

The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.

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Year:  1998        PMID: 9708362     DOI: 10.1080/07328319808005198

Source DB:  PubMed          Journal:  Nucleosides Nucleotides        ISSN: 0732-8311


  5 in total

Review 1.  Survey and summary: The applications of universal DNA base analogues.

Authors:  D Loakes
Journal:  Nucleic Acids Res       Date:  2001-06-15       Impact factor: 16.971

2.  Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution.

Authors:  Tuck Seng Wong; Kang Lan Tee; Berhard Hauer; Ulrich Schwaneberg
Journal:  Nucleic Acids Res       Date:  2004-02-10       Impact factor: 16.971

Review 3.  Non-natural nucleotides as probes for the mechanism and fidelity of DNA polymerases.

Authors:  Irene Lee; Anthony J Berdis
Journal:  Biochim Biophys Acta       Date:  2009-09-03

4.  Development of a 'clickable' non-natural nucleotide to visualize the replication of non-instructional DNA lesions.

Authors:  Edward A Motea; Irene Lee; Anthony J Berdis
Journal:  Nucleic Acids Res       Date:  2011-11-15       Impact factor: 16.971

5.  Modified Nucleotides as Substrates of Terminal Deoxynucleotidyl Transferase.

Authors:  Daiva Tauraitė; Jevgenija Jakubovska; Julija Dabužinskaitė; Maksim Bratchikov; Rolandas Meškys
Journal:  Molecules       Date:  2017-04-22       Impact factor: 4.411

  5 in total

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