| Literature DB >> 9708348 |
M Koizumi1, Y Ozawa, R Yagi, T Nishigaki, M Kaneko, S Oka, S Kimura, A Iwamoto, Y Komatsu, E Ohtsuka.
Abstract
Three ribozymes, a hairpin ribozyme (HR112) and two hammerhead ribozymes (RZ115 and RZ119) containing a 5'C(UUCG)G3' loop were designed to cleave the U5 region in the long terminal repeat (LTR) of HIV-1 RNA. The t1/2 values of chemically synthesized substrates mediated by three ribozymes were measured. The transformed CEM cells possessing these three ribozyme-encoding genes were challenged with a HIV-1IIIB strain, and two of these three ribozymes, HR112 and RZ119, were shown to possess strong anti-HIV-1 activity.Entities:
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Year: 1998 PMID： 9708348 DOI： 10.1080/07328319808005170
Source DB: PubMed Journal: Nucleosides Nucleotides ISSN： 0732-8311