Literature DB >> 9705524

Identification of novel simple sequence length polymorphisms (SSLPs) in mouse by interspersed repetitive element (IRE)-PCR.

J C Detter1, Q A Nguyen, S F Kingsmore.   

Abstract

Interspersed repetitive element (IRE)-PCR is a useful method for identification of novel human or mouse sequence tagged sites (STSs) from contigs of genomic clones. We describe the use of IRE-PCR with mouse B1 repetitive element primers to generate novel, PCR amplifiable, simple sequence length polymorphisms (SSLPs) from yeast artificial chromosome (YAC) clones containing regions of mouse chromosomes 13 and 14. Forty-two IRE-PCR products were cloned and sequenced from eight YACs. Of these, 29 clones contained multiple simple sequence repeat units. PCR analysis with primers derived from unique sequences flanking the simple sequence repeat units in seven clones showed all to be polymorphic between various mouse strains. This novel approach to SSLP identification represents an efficient method for saturating a genomic interval with polymorphic genetic markers that may expedite the positional cloning of genes for traits and diseases.

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Year:  1998        PMID： 9705524      PMCID： PMC147784          DOI： 10.1093/nar/26.17.4091

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

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Authors:  S F Kingsmore; M D Barbosa; Q A Nguyen; J A Ashley; S M Blaydes; V T Tchernev; J C Detter; M Lovett
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5.  Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1.

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Authors:  A S Krayev; D A Kramerov; K G Skryabin; A P Ryskov; A A Bayev; G P Georgiev
Journal:  Nucleic Acids Res       Date:  1980-03-25       Impact factor: 16.971

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Authors:  L McCarthy; K Hunter; L Schalkwyk; L Riba; S Anson; R Mott; W Newell; C Bruley; I Bar; E Ramu
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-06       Impact factor: 11.205
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Authors:  Chao-Jiang Gu; Alejandra Borjabad; Eran Hadas; Jennifer Kelschenbach; Boe-Hyun Kim; Wei Chao; Ottavio Arancio; Jin Suh; Bruce Polsky; JoEllyn McMillan; Benson Edagwa; Howard E Gendelman; Mary Jane Potash; David J Volsky
Journal:  PLoS Pathog       Date:  2018-06-07       Impact factor: 6.823
  3 in total

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