| Literature DB >> 9705176 |
E Beirnaert1, B Willems, M Peeters, A Bouckaert, L Heyndrickx, P Zhong, K Vereecken, S Coppens, D Davis, P Ndumbe, W Janssens, G van der Groen.
Abstract
An enzyme-linked immuno-sorbent assay (ELISA) for the detection of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIVcpz/SIVmnd) antigens was designed using immunoreagents from naturally infected individuals, and compared to the commercially available Vironostika HIV-1 Antigen Microelisa System (Organon Teknika). The in-house assay proved to be specific for HIV-1 isolates belonging to group M (A-H) and group O and for SIVcpz and SIVmnd isolates, but was less sensitive than the Vironostika HIV-1 Antigen Microelisa System, except for SIVmnd. For the strains belonging to HIV-2, SIVmac and SIVagm, the in-house assay could not detect antigen to an appreciable degree. This study shows that a considerably less expensive but sufficiently accurate HIV-1 antigen capture assay can be developed to monitor HIV-1 (group M and O), SIVcpv and SIVmnd antigen in the supernatants of virus cultures.Entities:
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Year: 1998 PMID： 9705176 DOI： 10.1016/s0166-0934(98)00044-5
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014