Literature DB >> 9704571

Parathyroid hormone-related protein expression and secretion in a skin organotypic culture system.

E A Blomme1, J R Werkmeister, H Zhou, V Kartsogiannis, C C Capen, T J Rosol.   

Abstract

Parathyroid hormone-related protein (PTHrP), an important factor in the pathogenesis of humoral hypercalcemia of malignancy, is produced by many normal tissues, including the epidermis, where it is thought to play a role in the regulation of keratinocyte growth and differentiation. Most in vitro studies of normal keratinocytes use monolayer cell cultures, which have limitations, including the inability to reproduce the stratified structure of the epidermis. The objective of this study was to investigate PTHrP production and secretion, and mRNA expression in skin organotypic cultures. The cultures consisted of an artificial dermis with differentiating keratinocytes grown at the air-liquid interface. Immunohistochemical assessment of cytokeratins 14 and 10/13, involucrin, and proliferative cell nuclear antigen (PCNA) demonstrated that keratinocytes differentiated in a manner similar to keratinocytes in normal epidermis. PTHrP expression was demonstrated in all viable layers of the epidermis, as well as in some fibroblasts of the collagen lattice by immunohistochemistry and in situ hybridization. Since most fibroblasts expressed alpha-smooth muscle actin, these cells were interpreted to be consistent with myofibroblasts. PTHrP expression by myofibroblasts suggests a possible role for PTHrP in the regulation of contractibility of these cells. PTHrP was also detected in conditioned media for 50 days. In conclusion, because of its superior tissue morphology and ability to induce organized keratinocyte differentiation, this culture system will be an excellent model to study the role of PTHrP in pathologic and physiologic processes involving the epidermis in vitro.

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Year:  1998        PMID: 9704571     DOI: 10.1385/ENDO:8:2:143

Source DB:  PubMed          Journal:  Endocrine        ISSN: 1355-008X            Impact factor:   3.633


  37 in total

1.  Reconstruction of the skin in three-dimensional collagen gel matrix culture.

Authors:  H Sugihara; S Toda; S Miyabara; Y Kusaba; Y Minami
Journal:  In Vitro Cell Dev Biol       Date:  1991-02

2.  Alpha-smooth muscle actin is expressed in a subpopulation of cultured and cloned fibroblasts and is modulated by gamma-interferon.

Authors:  A Desmoulière; L Rubbia-Brandt; A Abdiu; T Walz; A Macieira-Coelho; G Gabbiani
Journal:  Exp Cell Res       Date:  1992-07       Impact factor: 3.905

3.  Epidermal morphogenesis and induction of the 67 kD keratin polypeptide by culture of human keratinocytes at the liquid-air interface.

Authors:  D Asselineau; B Bernhard; C Bailly; M Darmon
Journal:  Exp Cell Res       Date:  1985-08       Impact factor: 3.905

4.  Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis.

Authors:  G N Van Muijen; S O Warnaar; M Ponec
Journal:  Exp Cell Res       Date:  1987-08       Impact factor: 3.905

5.  Cultured human fibroblasts and not cultured human keratinocytes express a PTH/PTHrP receptor mRNA.

Authors:  N M Hanafin; T C Chen; G Heinrich; G V Segre; M F Holick
Journal:  J Invest Dermatol       Date:  1995-07       Impact factor: 8.551

6.  A parathyroid hormone antagonist stimulates epidermal proliferation and hair growth in mice.

Authors:  M F Holick; S Ray; T C Chen; X Tian; K S Persons
Journal:  Proc Natl Acad Sci U S A       Date:  1994-08-16       Impact factor: 11.205

7.  Epithelial-specific gene expression during differentiation of stratified primary human keratinocyte cultures.

Authors:  J L Wilson; S C Dollard; L T Chow; T R Broker
Journal:  Cell Growth Differ       Date:  1992-08

8.  In situ hybridization to show sequential expression of osteoblast gene markers during bone formation in vivo.

Authors:  H Zhou; P Choong; R McCarthy; S T Chou; T J Martin; K W Ng
Journal:  J Bone Miner Res       Date:  1994-09       Impact factor: 6.741

9.  Lipid content and metabolism of human keratinocyte cultures grown at the air-medium interface.

Authors:  M L Williams; B E Brown; D J Monger; S Grayson; P M Elias
Journal:  J Cell Physiol       Date:  1988-07       Impact factor: 6.384

Review 10.  Involucrin and other markers of keratinocyte terminal differentiation.

Authors:  F M Watt
Journal:  J Invest Dermatol       Date:  1983-07       Impact factor: 8.551

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  1 in total

1.  Bone-invasive oral squamous cell carcinoma in cats: pathology and expression of parathyroid hormone-related protein.

Authors:  C K Martin; S H Tannehill-Gregg; T D Wolfe; T J Rosol
Journal:  Vet Pathol       Date:  2010-10-12       Impact factor: 2.221

  1 in total

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