| Literature DB >> 9701403 |
C Rosenberg1, R P van Gijlswijk, C B Vos, J Wiegant, C J Cornelisse, H J Tanke, A K Raap.
Abstract
Biotin deoxyuridine 5'-triphosphate (dUTP) and digoxigenin dUTP are the labels most commonly used in comparative genomic hybridization (CGH). The relative infrequent use of direct fluorochrome-labeled nucleotides in CGH is related to the lower sensitivity they provide. Here we report the evaluation of two fluorochrome-conjugated nucleotides that have not been previously used in CGH (lissamine-5-dUTP and fluorescein-N6dATP) and show that this direct label combination performs at least as well as the indirect biotin/digoxigenin pair.Entities:
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Year: 1998 PMID： 9701403
Source DB: PubMed Journal: Cytometry ISSN： 0196-4763