Literature DB >> 9701292

A new assay for tRNA aminoacylation kinetics.

A D Wolfson1, J A Pleiss, O C Uhlenbeck.   

Abstract

An improved quantitative assay for tRNA aminoacylation is presented based on charging of a nicked tRNA followed by separation of an aminoacylated 3'-fragment on an acidic denaturing polyacrylamide gel. Kinetic parameters of tRNA aminoacylation by Escherichia coli AlaRS obtained by the new method are in excellent agreement with those measured by the conventional method. This assay provides several advantages over the traditional methods of measuring tRNA aminoacylation: (1) the fraction of aminoacyl-tRNA is measured directly; (2) data can be obtained at saturating amino acid concentrations; and (3) the assay is significantly more sensitive.

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Year:  1998        PMID： 9701292      PMCID： PMC1369678          DOI： 10.1017/s1355838298980700

Source DB:  PubMed          Journal:  RNA        ISSN： 1355-8382            Impact factor:   4.942


  24 in total

1.  The energetic basis of specificity in the Eco RI endonuclease--DNA interaction.

Authors:  D R Lesser; M R Kurpiewski; L Jen-Jacobson
Journal:  Science       Date:  1990-11-09       Impact factor: 47.728

2.  Folding of circularly permuted transfer RNAs.

Authors:  T Pan; R R Gutell; O C Uhlenbeck
Journal:  Science       Date:  1991-11-29       Impact factor: 47.728

3.  Secondary structure in formylmethionine tRNA influences the site-directed cleavage of ribonuclease H using chimeric 2'-O-methyl oligodeoxyribonucleotides.

Authors:  Y Hayase; H Inoue; E Ohtsuka
Journal:  Biochemistry       Date:  1990-09-18       Impact factor: 3.162

Review 4.  tRNA identity: a hair of the dogma that bit us.

Authors:  M Yarus
Journal:  Cell       Date:  1988-12-02       Impact factor: 41.582

5.  Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro.

Authors:  J R Sampson; O C Uhlenbeck
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

6.  Interpretation of incomplete reactions in tRNA aminoacylation. Aminoacylation of yeast tRNA Val II with yeast valyl-tRNA synthetase.

Authors:  J Bonnet; J P Ebel
Journal:  Eur J Biochem       Date:  1972-12-04

7.  Kinetic techniques for the investigation of amino acid: tRNA ligases (aminoacyl-tRNA synthetases, amino acid activating enzymes).

Authors:  E A Eigner; R B Loftfield
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

8.  Ternary complexes of Escherichia coli aminoacyl-tRNAs with the elongation factor Tu and GTP: thermodynamic and structural studies.

Authors:  G Ott; M Schiesswohl; S Kiesewetter; C Förster; L Arnold; V A Erdmann; M Sprinzl
Journal:  Biochim Biophys Acta       Date:  1990-08-27

9.  Evidence that the 3' end of a tRNA binds to a site in the adenylate synthesis domain of an aminoacyl-tRNA synthetase.

Authors:  K Hill; P Schimmel
Journal:  Biochemistry       Date:  1989-03-21       Impact factor: 3.162

10.  Kinetic anticooperativity in pre-steady-state formation of tryptophanyl adenylate by tryptophanyl-tRNA synthetase from beef pancreas. A consequence of the tryptophan anticooperative binding.

Authors:  J P Mazat; M Merle; P V Graves; G Merault; J C Gandar; B Labouesse
Journal:  Eur J Biochem       Date:  1982-11-15
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  30 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  2002-04-30       Impact factor: 11.205

2.  Adaptation to tRNA acceptor stem structure by flexible adjustment in the catalytic domain of class I tRNA synthetases.

Authors:  Cuiping Liu; Jeffrey M Sanders; John M Pascal; Ya-Ming Hou
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3.  Kinetics of tRNA folding monitored by aminoacylation.

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Journal:  RNA       Date:  2012-01-27       Impact factor: 4.942

4.  Binding of misacylated tRNAs to the ribosomal A site.

Authors:  Taraka Dale; Olke C Uhlenbeck
Journal:  RNA       Date:  2005-11       Impact factor: 4.942

5.  Methods for kinetic and thermodynamic analysis of aminoacyl-tRNA synthetases.

Authors:  Christopher S Francklyn; Eric A First; John J Perona; Ya-Ming Hou
Journal:  Methods       Date:  2008-02       Impact factor: 3.608

6.  Aminoacylating urzymes challenge the RNA world hypothesis.

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Journal:  J Biol Chem       Date:  2013-07-18       Impact factor: 5.157

7.  Expanding the genetic code of Escherichia coli with phosphotyrosine.

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8.  Substrate interaction defects in histidyl-tRNA synthetase linked to dominant axonal peripheral neuropathy.

Authors:  Jamie A Abbott; Rebecca Meyer-Schuman; Vincenzo Lupo; Shawna Feely; Inès Mademan; Stephanie N Oprescu; Laurie B Griffin; M Antonia Alberti; Carlos Casasnovas; Sharon Aharoni; Lina Basel-Vanagaite; Stephan Züchner; Peter De Jonghe; Jonathan Baets; Michael E Shy; Carmen Espinós; Borries Demeler; Anthony Antonellis; Christopher Francklyn
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9.  The archaeal transamidosome for RNA-dependent glutamine biosynthesis.

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10.  Specificity of the ribosomal A site for aminoacyl-tRNAs.

Authors:  Taraka Dale; Richard P Fahlman; Mikołaj Olejniczak; Olke C Uhlenbeck
Journal:  Nucleic Acids Res       Date:  2009-01-07       Impact factor: 16.971
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