BACKGROUND: In recent studies, IL-1beta stimulated the production of IL-6 in human enterocytes. The heat shock response influences the production of inflammatory mediators in certain cell types. We tested the hypothesis that heat shock regulates IL-1beta-induced IL-6 production in human intestinal epithelial cells. MATERIALS AND METHODS: Cultured Caco-2 cells, a human intestinal epithelial cell line, were exposed to thermal heat shock at 43 degreesC for 1 h and recovered at 37 degreesC for 1 h. Cells were harvested for analysis of heat shock protein-70 (HSP-70) production by Western blotting. In other experiments, IL-1beta (0.5 ng/ml) was added following heat shock and recovery. IL-6 protein was measured in culture medium after 24 h by enzyme-linked immunosorbent assay and IL-6 messenger RNA (mRNA) levels were measured after 4 h by competitive reverse transcriptase polymerase chain reaction. RESULTS: Heat shock resulted in the production of HSP-70 and potentiated IL-1beta-induced IL-6 production. The response to heat shock was associated with increased IL-6 mRNA levels. CONCLUSIONS: The results suggest that IL-1beta-induced IL-6 production in human enterocytes is increased in association with the heat shock response. The biological role of heat shock-potentiated IL-6 production in the enterocyte remains to be determined. Copyright 1998 Academic Press.
BACKGROUND: In recent studies, IL-1beta stimulated the production of IL-6 in human enterocytes. The heat shock response influences the production of inflammatory mediators in certain cell types. We tested the hypothesis that heat shock regulates IL-1beta-induced IL-6 production in human intestinal epithelial cells. MATERIALS AND METHODS: Cultured Caco-2 cells, a human intestinal epithelial cell line, were exposed to thermal heat shock at 43 degreesC for 1 h and recovered at 37 degreesC for 1 h. Cells were harvested for analysis of heat shock protein-70 (HSP-70) production by Western blotting. In other experiments, IL-1beta (0.5 ng/ml) was added following heat shock and recovery. IL-6 protein was measured in culture medium after 24 h by enzyme-linked immunosorbent assay and IL-6 messenger RNA (mRNA) levels were measured after 4 h by competitive reverse transcriptase polymerase chain reaction. RESULTS: Heat shock resulted in the production of HSP-70 and potentiated IL-1beta-induced IL-6 production. The response to heat shock was associated with increased IL-6 mRNA levels. CONCLUSIONS: The results suggest that IL-1beta-induced IL-6 production in human enterocytes is increased in association with the heat shock response. The biological role of heat shock-potentiated IL-6 production in the enterocyte remains to be determined. Copyright 1998 Academic Press.
Authors: Steven S Welc; Neil A Phillips; Jose Oca-Cossio; Shannon M Wallet; Daniel L Chen; Thomas L Clanton Journal: Am J Physiol Cell Physiol Date: 2012-06-06 Impact factor: 4.249
Authors: Anita L Ray; Kiersten L Berggren; Sebastian Restrepo Cruz; Gregory N Gan; Ellen J Beswick Journal: Int J Cancer Date: 2017-12-12 Impact factor: 7.396
Authors: K Schlottmann; F-P Wachs; J Grossmann; D Vogl; M Maendel; W Falk; J Schölmerich; T Andus; G Rogler Journal: Int J Colorectal Dis Date: 2004-02-21 Impact factor: 2.571
Authors: Steven S Welc; Deborah A Morse; Alex J Mattingly; Orlando Laitano; Michelle A King; Thomas L Clanton Journal: PLoS One Date: 2016-02-12 Impact factor: 3.240