| Literature DB >> 9697316 |
H Oka1, Y Ito, S Yamada, T Kagami, J Hayakawa, K Harada, E Atsumi, M Suzuki, M Suzuki, H Odani, S Akahori, K Maeda, H Nakazawa, Y Ito.
Abstract
High-speed counter-current chromatography has been successfully applied to the separation of the lac dye components. A 25-mg quantity of the sample was separated using a two-phase solvent system composed of tert.-butyl methyl ether-n-butanol-acetonitrile-water (2:2:1:5). The fractions were analyzed by high-performance liquid chromatography and electrospray tandem mass spectrometry. The separation yielded 2.6 mg of 97.2% pure laccaic acid C, 9.5 mg of 98.1% pure laccaic acid A, 3.6 mg of 98.2% pure laccaic acid B, and 0.5 mg of a 95.0% pure anthraquinonedicarboxylic acid with a molecular mass of 360.Entities:
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Year: 1998 PMID： 9697316 DOI： 10.1016/s0021-9673(98)00311-2
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759