Properties of cattle bone powder-coated composite particles as high-performance and open column liquid chromatographic column packings.

Cattle bone powder (CBP) from natural resources was employed as a protein adsorbent instead of chemically synthesized hydroxyapatite (HA). Though a small amount of impurities was detected, CBP possessed a crystallinity similar to HA. Using CBP/40PE prepared from CBP and polyethylene beads (40 microns) by dry impact blending as an HPLC column packing, considerable correlation was observed between the elution concentrations of proteins and their pI. Such behavior was caused by the relatively large adsorption capacity for basic proteins. CBP/40PE could completely separate gamma-globulin from BSA also as an open column chromatographic support, under relatively low concentration.