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Literature DB >> 9696778

Analysis of the puc operon promoter from Rhodobacter capsulatus.

Abstract

Expression of the Rhodobacter capsulatus puc operon, which codes for structural polypeptides of the light-harvesting-II peripheral antenna complex, is highly regulated in response to alterations in oxygen tension and light intensity. To obtain an understanding of the puc promoter region we report the high-resolution 5' mapping of the puc mRNA transcriptional start site and DNA sequence analysis of the puc upstream regulatory sequence (pucURS). A sigma70-type promoter sequence was identified (pucP1) which has a high degree of sequence similarity with carotenoid and bacteriochlorophyll biosynthesis promoters. Inspection of the DNA sequence also indicated the presence of two CrtJ and four integration host factor (IHF) binding sites. Transcriptional fusions of the pucURS fused to lacZ also confirmed that puc promoter activity is regulated by the transcriptional regulators IHF, CrtJ, and RegA. Gel retardation analysis using cell extracts indicates that mutations in IHF and RegA disrupt protein binding to DNA fragments containing the pucURS.

Entities: Chemical Disease Species

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Year: 1998 PMID： 9696778 PMCID： PMC107426

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

39 in total

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9 in total

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Journal: Microbiol Mol Biol Rev Date: 2004-06 Impact factor: 11.056

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6. Taxonomic Re-Evaluation and Genomic Comparison of Novel Extracellular Electron Uptake-Capable Rhodovulum&nbsp;visakhapatnamense and Rhodovulum sulfidophilum Isolates.

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Journal: Microorganisms Date: 2022-06-16

7. Structural Analyses of CrtJ and Its B₁₂-Binding Co-Regulators SAerR and LAerR from the Purple Photosynthetic Bacterium Rhodobacter capsulatus.

Authors: Vladimira Dragnea; Giovanni Gonzalez-Gutierrez; Carl E Bauer
Journal: Microorganisms Date: 2022-04-27

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Authors: Gregory S Orf; Rafael G Saer; Dariusz M Niedzwiedzki; Hao Zhang; Chelsea L McIntosh; Jason W Schultz; Liviu M Mirica; Robert E Blankenship
Journal: Proc Natl Acad Sci U S A Date: 2016-06-22 Impact factor: 11.205

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9 in total