| Literature DB >> 9693055 |
C van den Bos1, A Rammes, T Vogl, R Boynton, J Zaia, C Sorg, J Roth.
Abstract
A method is described for purification of P6, MRP8, and MRP14, three calcium-binding proteins assigned to the S100 protein family. The purification procedure included preparation of human granulocytes, ammonium sulfate precipitation, and anion-exchange chromatography and resulted in the copurification of P6, MRP8, and MRP14. Individual proteins were separated by either preparative isoelectric focusing or preparative SDS-PAGE. The procedure was carried out in the course of 4 days and yielded several milligrams of essentially pure P6, MRP8, and MRP14 in either native or denatured form. Copyright 1998 Academic Press.Entities:
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Year: 1998 PMID： 9693055 DOI： 10.1006/prep.1998.0917
Source DB: PubMed Journal: Protein Expr Purif ISSN： 1046-5928 Impact factor: 1.650