Determination of minocycline in human plasma by high-performance liquid chromatography with UV detection after liquid-liquid extraction.

A sensitive and specific high-performance liquid chromatographic method was developed and validated for the determination of minocycline in human plasma. The method uses liquid-liquid extraction, reextraction and HPLC with UV detection. The assay shows linearity in the tested range of 28-3533 ng/ml with a limit of quantification of 30 ng/ml. The inter-day precision was found to be +/-3.84 to +/-6.57% (R.S.D.) in the range of 148 to 2743 ng/ml. The method was successfully applied to pharmacokinetic studies.