Border disease of sheep and goats.

Border disease (BD) is a congenital virus disease of sheep and goats first reported in 1959 from the border region of England and Wales. BD virus (BDV) is a pestivirus in the genus Flaviviridae and is closely related to classical swine fever virus and bovine virus diarrhoea virus (BVDV). Nearly all isolates of BDV are non-cytopathogenic (ncp) in cell culture. There are no defined serotypes but pestiviruses isolated from sheep exhibit considerable antigenic diversity and three distinct antigenic groups have been identified. Distribution of the virus is worldwide. Prevalence rates vary in sheep from 5 to 50% between countries and from region-to-region within countries. The disease in goats is rare and characterized by abortion. Clinical signs in sheep include barren ewes, abortions, stillbirths and the birth of small weak lambs. Affected lambs can show tremor, abnormal body conformation and hairy fleeces (so-called 'hairy-shaker' or 'fuzzy' lambs). Vertical transmission plays an important role in the epidemiology of the disease. Infection of fetuses can result in the birth of persistently infected (PI) lambs. These PI lambs are viraemic, antibody negative and constantly excrete virus. The virus spreads from sheep to sheep with PI animals being the most potent source of infection. Apparently healthy PI sheep resulting from congenital infection can be identified by direct detection of viral antigen or viral RNA in leukocytes or by isolation of ncp virus from blood or serum in laboratory cell cultures. Isolation of virus is unreliable in lambs younger than 2 months old that have received colostral antibody. The isolation of virus from tissues of aborted or stillborn lambs is difficult but tissues from PI sheep contain easily detectable levels of virus. To detect the growth of virus in cell cultures it is essential to use an immune-labelling method. Acute infection is usually subclinical and viraemia is transient and difficult to detect. Sheep may also be infected following close contact with cattle excreting the closely related BVDV.