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Literature DB >> 9688584

Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS.

N J Clarke¹, A J Tomlinson, Y Ohyagi, S Younkin, S Naylor.

Abstract

A quantitative method for detection of amyloid beta peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of A beta 1-40 and A beta 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for A beta 1-40 and A beta 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other A beta peptide species that may also play a role in the onset of AD in humans.

Entities: Disease Gene Species

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Year: 1998 PMID： 9688584 DOI： 10.1016/s0014-5793(98)00706-6

Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124

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Cited

9 in total

Review 1. Guinea pigs as a nontransgenic model for APP processing in vitro and in vivo.

Authors: Mike Beck; Volker Bigl; Steffen Rossner
Journal: Neurochem Res Date: 2003-04 Impact factor: 3.996

2. 4-hexylresorcinol-induced protein expression changes in human umbilical cord vein endothelial cells as determined by immunoprecipitation high-performance liquid chromatography.

Authors: Yeon Sook Kim; Dae Won Kim; Seong-Gon Kim; Suk Keun Lee
Journal: PLoS One Date: 2020-12-15 Impact factor: 3.240

3. Development of a magnetic immunosorbent for on-chip preconcentration of amyloid β isoforms: Representatives of Alzheimer's disease biomarkers.

Authors: Zuzana Svobodova; Mohamad Reza Mohamadi; Barbora Jankovicova; Hermann Esselmann; Romain Verpillot; Markus Otto; Myriam Taverna; Jens Wiltfang; Jean-Louis Viovy; Zuzana Bilkova
Journal: Biomicrofluidics Date: 2012-05-24 Impact factor: 2.800

9. γ-Secretase-Dependent Proteolysis of Transmembrane Domain of Amyloid Precursor Protein: Successive Tri- and Tetrapeptide Release in Amyloid β-Protein Production.

Authors: Mako Takami; Satoru Funamoto
Journal: Int J Alzheimers Dis Date: 2012-12-31

9 in total

Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS.

Review 1. Guinea pigs as a nontransgenic model for APP processing in vitro and in vivo.

2. 4-hexylresorcinol-induced protein expression changes in human umbilical cord vein endothelial cells as determined by immunoprecipitation high-performance liquid chromatography.

3. Development of a magnetic immunosorbent for on-chip preconcentration of amyloid β isoforms: Representatives of Alzheimer's disease biomarkers.

4. Longer forms of amyloid beta protein: implications for the mechanism of intramembrane cleavage by gamma-secretase.

5. Colorimetric cellulose-based test-strip for rapid detection of amyloid β-42.

6. Integration of a miniature quartz crystal microbalance with a microfluidic chip for amyloid beta-Aβ42 quantitation.

7. 4-Hexylresorcinol-in duced angiogenesis potential in human endothelial cells.

8. Extensive protein expression changes induced by pamidronate in RAW 264.7 cells as determined by IP-HPLC.

9. γ-Secretase-Dependent Proteolysis of Transmembrane Domain of Amyloid Precursor Protein: Successive Tri- and Tetrapeptide Release in Amyloid β-Protein Production.