Literature DB >> 9688524

Structural analysis of the amplified IFN-beta and DHFR genes in a Chinese hamster ovary cell line using multicolour FISH analysis.

C Dixkens1, G Posseckert, T Keller, H Hameister.   

Abstract

Multicolour FISH was used to get insight into the structural arrangement of a homogeneously staining region which bears the co-transfected and subsequently co-amplified IFN-beta and DHFR genes in a CHO cell line. On metaphase chromosomes an arrangement of multiple bands with regular spacing is revealed. On extended chromatin fibres a cluster of directly repeated and interspersed IFN-beta and DHFR genes is visible. Up to three clusters were found arranged in tandem. The different chromosomal mechanisms leading to gene amplification are discussed.

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Year:  1998        PMID： 9688524     DOI： 10.1023/a:1009231110030

Source DB:  PubMed          Journal:  Chromosome Res        ISSN： 0967-3849            Impact factor:   5.239


  10 in total

1.  The evolution of the amplified adenylate deaminase 2 domains in Chinese hamster cells suggests the sequential operation of different mechanisms of DNA amplification.

Authors:  F Toledo; K A Smith; G Buttin; M Debatisse
Journal:  Mutat Res       Date:  1992-05       Impact factor: 2.433

Review 2.  Amplification of the dihydrofolate reductase gene in methotrexate-resistant Chinese hamster cells.

Authors:  J L Hamlin
Journal:  Mutat Res       Date:  1992-05       Impact factor: 2.433

Review 3.  Recent progress in understanding mechanisms of mammalian DNA amplification.

Authors:  G R Stark; M Debatisse; E Giulotto; G M Wahl
Journal:  Cell       Date:  1989-06-16       Impact factor: 41.582

4.  High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.

Authors:  P Lichter; C J Tang; K Call; G Hermanson; G A Evans; D Housman; D C Ward
Journal:  Science       Date:  1990-01-05       Impact factor: 47.728

5.  Evolution and stability of chromosomal DNA coamplified with the CAD gene.

Authors:  I Saito; R Groves; E Giulotto; M Rolfe; G R Stark
Journal:  Mol Cell Biol       Date:  1989-06       Impact factor: 4.272

Review 6.  Gene amplification in cultured cells.

Authors:  R T Schimke
Journal:  J Biol Chem       Date:  1988-05-05       Impact factor: 5.157

7.  Sister chromatid fusion initiates amplification of the dihydrofolate reductase gene in Chinese hamster cells.

Authors:  C Ma; S Martin; B Trask; J L Hamlin
Journal:  Genes Dev       Date:  1993-04       Impact factor: 11.361

8.  Early dihydrofolate reductase gene amplification events in CHO cells usually occur on the same chromosome arm as the original locus.

Authors:  B J Trask; J L Hamlin
Journal:  Genes Dev       Date:  1989-12       Impact factor: 11.361

9.  Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.

Authors:  G Urlaub; L A Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

10.  Released chromatin: linearized DNA for high resolution fluorescence in situ hybridization.

Authors:  G Senger; T A Jones; H Fidlerová; P Sanséau; J Trowsdale; M Duff; D Sheer
Journal:  Hum Mol Genet       Date:  1994-08       Impact factor: 6.150
  10 in total

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