Literature DB >> 9684646

A semisynthetic fetal calf serum-free liquid medium for in vitro cultivation of Leishmania promastigotes.

S A Ali1, J Iqbal, B Ahmad, M Masoom.   

Abstract

A semisynthetic, autoclavable, liquid medium that does not require inclusion of fetal calf serum (FCS) for the successful cultivation of Leishmania promastigotes in vitro is described. The parasites were grown in commercially available medium 199 supplemented with 10% FCS and 2% mammalian urine and the growth was compared with the same medium without FCS. The growth of the parasites in the semidefined medium without FCS was found to be as good as or slightly better that with medium 199 supplemented with FCS.

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Year:  1998        PMID： 9684646     DOI： 10.4269/ajtmh.1998.59.163

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN： 0002-9637            Impact factor:   2.345


  9 in total

1.  LB broth-lyophilized Rabbit serum (LLR) as a new and suitable culture medium for cultivation of promastigotes of Leishmania major.

Authors:  Vahid Nasiri; Abdolhossein Dalimi; Fatemeh Ghaffarifar
Journal:  J Parasit Dis       Date:  2016-05-26

Review 2.  Recombinant protein expression in Leishmania tarentolae.

Authors:  Giancarlo Basile; Manuela Peticca
Journal:  Mol Biotechnol       Date:  2009-11       Impact factor: 2.695

3.  Low-cost liquid medium for in vitro cultivation of Leishmania parasites in low-income countries.

Authors:  Geremew Tasew; Amha Kebede; Dawit Wolday; Endalamaw Gadisa; Sven Britton; Liv Eidsmo; Hannah Akuffo
Journal:  Glob Health Action       Date:  2009-10-22       Impact factor: 2.640

4.  Milk of cow (Bos taurus), buffalo (Bubalus bubalis), and goat (Capra hircus): a better alternative than fetal bovine serum in media for primary isolation, in vitro cultivation, and maintenance of Leishmania donovani promastigotes.

Authors:  M Muniaraj; C S Lal; S Kumar; P K Sinha; P Das
Journal:  J Clin Microbiol       Date:  2007-02-07       Impact factor: 5.948

Review 5.  Cultivation of clinically significant hemoflagellates.

Authors:  Frederick L Schuster; James J Sullivan
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132

6.  Human urine stimulates in vitro growth of Trypanosoma cruzi and Trypanosoma rangeli.

Authors:  Keila A M Ferreira; Paulo E S Lemos-Júnior; Eliane Lages-Silva; Luis E Ramírez; André L Pedrosa
Journal:  Parasitol Res       Date:  2007-07-15       Impact factor: 2.289

7.  Effects of sheep and mouse urine on the growth pattern of Leishmania major promastigotes.

Authors:  Vahid Nasiri; Gholamreza Karimi; Abdolhossein Dalimi; Habibollah Paykari; Fatemeh Ghaffarifar
Journal:  Biomed Res Int       Date:  2013-07-15       Impact factor: 3.411

8.  Liver infusion tryptose (LIT): the best choice for growth, viability, and infectivity of Leishmania infantum parasites.

Authors:  Ana Flávia Pereira Costa; Rory Cristiane Fortes de Brito; Lívia Mendes Carvalho; Jamille Mirelle de Oliveira Cardoso; Paula Melo de Abreu Vieira; Alexandre Barbosa Reis; Rodrigo Dian de Oliveira Aguiar-Soares; Bruno Mendes Roatt
Journal:  Parasitol Res       Date:  2020-10-09       Impact factor: 2.289

9.  Leishmania Parasite: the Impact of New Serum-Free Medium as an Alternative for Fetal Bovine Serum

Authors:  Sima Habibzadeh; Delaram Doroud; Tahereh Taheri; Negar Seyed; Sima Rafati
Journal:  Iran Biomed J       Date:  2021-08-29
  9 in total

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