Effects of a vegetables-fruit mixture on liver and colonic 1,2-dimethylhydrazine-metabolizing enzyme activities in rats fed low- or high-fat diets.

The effects of a vegetables-fruit mixture (19.5% w/w) were studied on hepatic (h) and colonic (c) 1,2-dimethylhydrazine (DMH)-metabolizing enzyme activities (ethoxy- and pentoxyresorufine-O-deethylation, NDMA-demethylase, glutathione-S-transferase, UDP-glucuronyltransferase) in rats fed low- or high-fat diets (20 or 40 energy%). A vegetables-fruit mixture added to the diets resulted in altered enzyme activities in animals either treated or not treated with DMH. Remarkably, the vegetables-fruit mixture given to DMH-treated rats decreased glutathione-S-transferase (h) and increased NDMA-demethylase activities (c), whereas the mixture given to controls increased glutathione-S-transferase (h) and decreased NDMA-demethylase activities (c). The high-fat diets only modulated enzyme activities in animals not treated with DMH.