PURPOSE OF STUDY: IL-10 may explain the paradox between immunodeficiency and oversecretion of cytokines in chronic haemodialysis (HD) patients. We analysed the secretion of IL-10 by PBMC and the expression of IL-10 mRNA in 10 long-term HD patients (108-276 months), 10 short-term HD patients (3-18 months), and 10 healthy controls. RESULTS: Spontaneous IL-10 secretion was higher in HD patients than in controls (15 pg/ml vs 2 pg/ml, P = 0.004). It was detected in 13 of 20 patients and in 1 of 10 controls (P = 0.01). IL-10 mRNA expression was also higher in HD patients than in controls. Spontaneous secretions of IL-10 and IL-6 were positively correlated in patients. IL-10 secretion in response to LPS was higher than the upper limit of control range in 4 of 10 long-term HD patients and in no short-term HD patients (P = 0.04). IL-10 mRNA expression was also higher in long-term than in short-term HD patients. CONCLUSIONS: This study demonstrates that IL-10 is spontaneously synthesized and secreted in HD patients, supporting an immunomodulating role in this setting. The greater IL-10-producing capacity in long-term HD patients indicates a chronic effect of haemodialysis on PBMC responsiveness.
PURPOSE OF STUDY: IL-10 may explain the paradox between immunodeficiency and oversecretion of cytokines in chronic haemodialysis (HD) patients. We analysed the secretion of IL-10 by PBMC and the expression of IL-10 mRNA in 10 long-term HDpatients (108-276 months), 10 short-term HDpatients (3-18 months), and 10 healthy controls. RESULTS: Spontaneous IL-10 secretion was higher in HDpatients than in controls (15 pg/ml vs 2 pg/ml, P = 0.004). It was detected in 13 of 20 patients and in 1 of 10 controls (P = 0.01). IL-10 mRNA expression was also higher in HDpatients than in controls. Spontaneous secretions of IL-10 and IL-6 were positively correlated in patients. IL-10 secretion in response to LPS was higher than the upper limit of control range in 4 of 10 long-term HDpatients and in no short-term HDpatients (P = 0.04). IL-10 mRNA expression was also higher in long-term than in short-term HDpatients. CONCLUSIONS: This study demonstrates that IL-10 is spontaneously synthesized and secreted in HDpatients, supporting an immunomodulating role in this setting. The greater IL-10-producing capacity in long-term HDpatients indicates a chronic effect of haemodialysis on PBMC responsiveness.
Authors: Ana Andres-Hernando; Belda Dursun; Christopher Altmann; Nilesh Ahuja; Zhibin He; Rhea Bhargava; Charles E Edelstein; Alkesh Jani; Thomas S Hoke; Christina Klein; Sarah Faubel Journal: Nephrol Dial Transplant Date: 2012-07-09 Impact factor: 5.992