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Literature DB >> 967696

Some properties of tobacco protoplast chromatin.

C Gigot, G Philipps, A Nicolaieff, L Hirth.

Abstract

Chromatin was prepared from tobacco-leaf protoplasts. Its solubility in increasing molarities of NaCl was studied and the structure of the soluble fraction observed by electron microscopy. We demonstrate that in plants, the DNA and histones are associated in beaded structures similar to those called omicron-bodies or nucleosomes in animal chromatin. The nucleosomes were associated with DNA in either compact or extended forms. The compact arrangement was predominant in the fraction solubilized between 0.1 and 0.4 M NaCl. The extended form, present at 0.5 and 0.6 M NaCl. showed DNA filaments of various lengths interspacing the nucleosomes. At these ionic strengths ring structures were present, associated with the DNA. At 0.7 M NaCl and above, only DNA filaments were present, occasionally associated with big rings, and nucleosomes were compoetely dissociated. Free DNA molecules were present at all ionic strengths used. The possible origin and significance of the rings are discussed.

Entities: Chemical Species

Mesh：

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Year: 1976 PMID： 967696 PMCID： PMC343087 DOI： 10.1093/nar/3.9.2315

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

24 in total

7. Electron microscopic studies of the different topological forms of the cauliflower mosaic virus DNA: knotted encapsidated DNA and nuclear minichromosome.

Authors: J Ménissier; G de Murcia; G Lebeurier; L Hirth
Journal: EMBO J Date: 1983 Impact factor: 11.598

7 in total

Some properties of tobacco protoplast chromatin.

1. Studies on chromatin. II. Isolation and characterization of chromatin subunits.

2. Isolation and characterization of the histones from cycad pollen.

3. Protein synthesis in tobacco protoplasts infected with tobacco mosaic virus.

4. Subunit structure of chromatin is the same in plants and animals.

5. Selective dissociation of histones from calf thymus nucleoprotein.

6. Electron microscopy of chromatin subunit particles.

7. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease.

8. Preparation of chromatin. Variation in the template properties of chromatin dependent on the method of perparation.

9. A new preparation method for dark-field electron microscopy of biomacromolecules.

10. High resolution acrylamide gel electrophoresis of histones.

1. Analysis of DNA associated with nucleosomes in pea chromatin.

2. Properties of condensed chromatin in barley nuclei.

3. DNA associated with nucleosomes in plants.

4. The helical model of the nucleosome core.

5. Asymmetric lateral distribution of unshielded phosphate groups in nucleosomal DNA and its role in DNA bending.

6. In-vitro transcription in tobacco chromatin.

7. Electron microscopic studies of the different topological forms of the cauliflower mosaic virus DNA: knotted encapsidated DNA and nuclear minichromosome.