OBJECTIVE: We hypothesized that human milk oligosaccharides (HMO) would not be digested and absorbed in the infant small intestine. The purpose of the study was to quantify the extent of digestion by using the lactulose breath hydrogen test. STUDY DESIGN: Twenty-four healthy, breast-fed infants were studied in the home setting. Eight infants (mean age 5.2 months) who had a positive breath hydrogen response (rise > 20 ppm) to the unabsorbable sugar lactulose were given an equivalent load of HMO (0.7 to 1.0 gm/kg body weight) the following week. The breath hydrogen response to the HMO load was compared with that after lactulose by using a paired t test. RESULTS: In seven of the eight infants, a large proportion of the HMO load reached the large intestine and was fermented. In these infants, the mean +/- SEM area under the breath hydrogen curve after HMO (5135 +/- 1148 ppm.4h) was not significantly different from that after lactulose (4949 +/- 1278 ppm.4h, p = 0.7s. CONCLUSIONS: This study suggests that HMO resist digestion in the small intestine of most breast-fed infants and undergo fermentation in the colon. HMO may therefore be the source of breath hydrogen in breast-fed infants.
OBJECTIVE: We hypothesized that human milk oligosaccharides (HMO) would not be digested and absorbed in the infant small intestine. The purpose of the study was to quantify the extent of digestion by using the lactulose breath hydrogen test. STUDY DESIGN: Twenty-four healthy, breast-fed infants were studied in the home setting. Eight infants (mean age 5.2 months) who had a positive breath hydrogen response (rise > 20 ppm) to the unabsorbable sugarlactulose were given an equivalent load of HMO (0.7 to 1.0 gm/kg body weight) the following week. The breath hydrogen response to the HMO load was compared with that after lactulose by using a paired t test. RESULTS: In seven of the eight infants, a large proportion of the HMO load reached the large intestine and was fermented. In these infants, the mean +/- SEM area under the breath hydrogen curve after HMO (5135 +/- 1148 ppm.4h) was not significantly different from that after lactulose (4949 +/- 1278 ppm.4h, p = 0.7s. CONCLUSIONS: This study suggests that HMO resist digestion in the small intestine of most breast-fed infants and undergo fermentation in the colon. HMO may therefore be the source of breath hydrogen in breast-fed infants.
Authors: Bryan Zabel; Christian Clement Yde; Paige Roos; Jørn Marcussen; Henrik Max Jensen; Krista Salli; Johanna Hirvonen; Arthur C Ouwehand; Wesley Morovic Journal: Sci Rep Date: 2019-05-28 Impact factor: 4.379
Authors: Dominique Turck; Jacqueline Castenmiller; Stefaan De Henauw; Karen Ildico Hirsch-Ernst; John Kearney; Alexandre Maciuk; Inge Mangelsdorf; Harry J McArdle; Androniki Naska; Carmen Pelaez; Kristina Pentieva; Alfonso Siani; Frank Thies; Sophia Tsabouri; Marco Vinceti; Francesco Cubadda; Thomas Frenzel; Marina Heinonen; Rosangela Marchelli; Monika Neuhäuser-Berthold; Morten Poulsen; Miguel Prieto Maradona; Josef Rudolf Schlatter; Henk van Loveren; Paolo Colombo; Helle Katrine Knutsen Journal: EFSA J Date: 2021-06-30
Authors: Olaf Perdijk; Marloes van Splunter; Huub F J Savelkoul; Sylvia Brugman; R J Joost van Neerven Journal: Front Immunol Date: 2018-02-12 Impact factor: 7.561