| Literature DB >> 9671761 |
G Zanusso1, D Liu, S Ferrari, I Hegyi, X Yin, A Aguzzi, S Hornemann, S Liemann, R Glockshuber, J C Manson, P Brown, R B Petersen, P Gambetti, M S Sy.
Abstract
By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.Entities:
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Year: 1998 PMID： 9671761 PMCID： PMC21159 DOI： 10.1073/pnas.95.15.8812
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205