Regulation of HSF1 activation and Hsp expression in mouse tissues under physiological stress conditions.

The studies described above illustrate several important points concerning the study of the cellular stress response. First, they indicate that there are important differences between the nature of the stress response observed in tumor cell lines and that of primary cells of mouse tissues. Therefore, caution should be exercised to avoid overextending and overinterpreting the results of studies using these cell lines until evidence can be obtained from in vivo studies on intact animals or primary cells of tissues. Second, they reveal the existence of cell-type-dependent differences between cell types of the same organism, thus potentially calling into question the supposed universal cytoprotective nature of the cellular stress response. Future studies can address this question by determining the underlying mechanisms that regulate these cell-type-dependent differences in the stress response, and by determining whether there are biological reasons that some cell types exhibit differences in either the temperature set-point or magnitude of the stress response.