Literature DB >> 9666006

5' nuclease PCR assay to detect Yersinia pestis.

J A Higgins1, J Ezzell, B J Hinnebusch, M Shipley, E A Henchal, M S Ibrahim.   

Abstract

The 5' nuclease PCR assay uses a fluorescently labeled oligonucleotide probe (TaqMan) to rapidly detect and quantitate DNA templates in clinical samples. We developed a 5' nuclease PCR assay targeting the plasminogen activator gene (pla) of Yersinia pestis. The assay is species specific, with a detection threshold of 2.1 x 10(5) copies of the pla target or 1.6 pg of total cell DNA. The assay detected Y. pestis in experimentally infected Xenopsylla cheopis fleas and in experimentally infected monkey blood and oropharyngeal swabs. The TaqMan assay is simple to perform and rapid and shows promise as a future field-adaptable technique.

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Year:  1998        PMID： 9666006      PMCID： PMC105032     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  A novel method for real time quantitative RT-PCR.

Authors:  U E Gibson; C A Heid; P M Williams
Journal:  Genome Res       Date:  1996-10       Impact factor: 9.043

2.  The potential of 5' nuclease PCR for detecting a single-base polymorphism in Orthopoxvirus.

Authors:  M S Ibrahim; J J Esposito; P B Jahrling; R S Lofts
Journal:  Mol Cell Probes       Date:  1997-04       Impact factor: 2.365

3.  Detection of Rift Valley fever virus in mosquitoes by RT-PCR.

Authors:  M S Ibrahim; M J Turell; F K Knauert; R S Lofts
Journal:  Mol Cell Probes       Date:  1997-02       Impact factor: 2.365

4.  The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities.

Authors:  S Chen; A Yee; M Griffiths; C Larkin; C T Yamashiro; R Behari; C Paszko-Kolva; K Rahn; S A De Grandis
Journal:  Int J Food Microbiol       Date:  1997-04-15       Impact factor: 5.277

5.  Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Authors:  T Krishnamurthy; P L Ross; U Rajamani
Journal:  Rapid Commun Mass Spectrom       Date:  1996       Impact factor: 2.419

6.  Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor.

Authors:  L K Cao; G P Anderson; F S Ligler; J Ezzell
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

7.  Pathology of experimental pneumonic plague produced by fraction 1-positive and fraction 1-negative Yersinia pestis in African green monkeys (Cercopithecus aethiops).

Authors:  K J Davis; D L Fritz; M L Pitt; S L Welkos; P L Worsham; A M Friedlander
Journal:  Arch Pathol Lab Med       Date:  1996-02       Impact factor: 5.534

8.  Detection and identification of Yersinia pestis by polymerase chain reaction (PCR) using multiplex primers.

Authors:  H Tsukano; K Itoh; S Suzuki; H Watanabe
Journal:  Microbiol Immunol       Date:  1996       Impact factor: 1.955

9.  Role of the Yersinia pestis hemin storage (hms) locus in the transmission of plague by fleas.

Authors:  B J Hinnebusch; R D Perry; T G Schwan
Journal:  Science       Date:  1996-07-19       Impact factor: 47.728

10.  Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes.

Authors:  H A Bassler; S J Flood; K J Livak; J Marmaro; R Knorr; C A Batt
Journal:  Appl Environ Microbiol       Date:  1995-10       Impact factor: 4.792
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  27 in total

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Authors:  G E Killgore; B Holloway; F C Tenover
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

2.  Comparison of methods of extracting Salmonella enterica serovar Enteritidis DNA from environmental substrates and quantification of organisms by using a general internal procedural control.

Authors:  M M Klerks; A H C van Bruggen; C Zijlstra; M Donnikov
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

3.  Quantitative real-time polymerase chain reaction for the core facility using TaqMan and the Perkin-Elmer/Applied Biosystems Division 7700 Sequence Detector.

Authors:  D S Grove
Journal:  J Biomol Tech       Date:  1999-03

4.  Evaluation of 5' nuclease assay for detection of Actinobacillus pleuropneumoniae.

Authors:  O Angen; J Jensen; D T Lavritsen
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

5.  Rapid, quantitative PCR monitoring of growth of Clostridium botulinum type E in modified-atmosphere-packaged fish.

Authors:  B Kimura; S Kawasaki; H Nakano; T Fujii
Journal:  Appl Environ Microbiol       Date:  2001-01       Impact factor: 4.792

6.  Automated 5' nuclease PCR assay for identification of Salmonella enterica.

Authors:  J Hoorfar; P Ahrens; P Rådström
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

7.  Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks.

Authors:  N Pusterla; J B Huder; C M Leutenegger; U Braun; J E Madigan; H Lutz
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

8.  Rapid and sensitive detection of Yersinia pestis using amplification of plague diagnostic bacteriophages monitored by real-time PCR.

Authors:  Kirill V Sergueev; Yunxiu He; Richard H Borschel; Mikeljon P Nikolich; Andrey A Filippov
Journal:  PLoS One       Date:  2010-06-28       Impact factor: 3.240

9.  Ambient stable quantitative PCR reagents for the detection of Yersinia pestis.

Authors:  Shi Qu; Qinghai Shi; Lei Zhou; Zhaobiao Guo; Dongsheng Zhou; Junhui Zhai; Ruifu Yang
Journal:  PLoS Negl Trop Dis       Date:  2010-03-09

10.  Comparative analysis of the Schleicher and Schuell IsoCode Stix DNA isolation device and the Qiagen QIAamp DNA Mini Kit.

Authors:  Susan R Coyne; Philip D Craw; David A Norwood; Melanie P Ulrich
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948
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