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Literature DB >> 9661195

The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions.

Abstract

The analysis of macromolecular interactions is an essential element of biomedical research. Flow cytometry is uniquely capable of making sensitive and quantitative measurements of molecular interactions. These measurements can be made in real time with subsecond kinetic resolution using purified biomolecules or living cells. Combined with automated sample handling, these features make flow cytometry a versatile and robust technology for the analysis of molecular interactions.

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Year: 1998 PMID： 9661195 DOI： 10.1038/nbt0798-633

Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908

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Cited

23 in total

1. FRET detection of cellular alpha4-integrin conformational activation.

Authors: Alexandre Chigaev; Tione Buranda; Denise C Dwyer; Eric R Prossnitz; Larry A Sklar
Journal: Biophys J Date: 2003-12 Impact factor: 4.033

2. A differential cell capture assay for evaluating antibody interactions with cell surface targets.

Authors: David J Sherman; Vania E Kenanova; Eric J Lepin; Katelyn E McCabe; Ken-Ichiro Kamei; Minori Ohashi; Shutao Wang; Hsian-Rong Tseng; Anna M Wu; Christian P Behrenbruch
Journal: Anal Biochem Date: 2010-02-21 Impact factor: 3.365

Review 3. Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems.

Authors: John P Nolan; Francis Mandy
Journal: Cytometry A Date: 2006-05 Impact factor: 4.355

4. Fluorescent DNA nanotags: supramolecular fluorescent labels based on intercalating dye arrays assembled on nanostructured DNA templates.

Authors: Andrea L Benvin; Yehuda Creeger; Gregory W Fisher; Byron Ballou; Alan S Waggoner; Bruce A Armitage
Journal: J Am Chem Soc Date: 2007-01-26 Impact factor: 15.419

5. Continuous inertial focusing, ordering, and separation of particles in microchannels.

Authors: Dino Di Carlo; Daniel Irimia; Ronald G Tompkins; Mehmet Toner
Journal: Proc Natl Acad Sci U S A Date: 2007-11-19 Impact factor: 11.205

6. The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy.

Authors: Yang Wu; Samuel K Campos; Gabriel P Lopez; Michelle A Ozbun; Larry A Sklar; Tione Buranda
Journal: Anal Biochem Date: 2007-02-13 Impact factor: 3.365

The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions.

1. FRET detection of cellular alpha4-integrin conformational activation.

2. A differential cell capture assay for evaluating antibody interactions with cell surface targets.

Review 3. Multiplexed and microparticle-based analyses: quantitative tools for the large-scale analysis of biological systems.

4. Fluorescent DNA nanotags: supramolecular fluorescent labels based on intercalating dye arrays assembled on nanostructured DNA templates.

5. Continuous inertial focusing, ordering, and separation of particles in microchannels.

6. The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy.

Review 7. New tools and new biology: recent miniaturized systems for molecular and cellular biology.

8. Single-cell attachment and culture method using a photochemical reaction in a closed microfluidic system.

9. A low-cost, plug-and-play inertial microfluidic helical capillary device for high-throughput flow cytometry.

10. Single stream inertial focusing in a straight microchannel.