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Literature DB >> 9660945

Human Smad3 and Smad4 are sequence-specific transcription activators.

L Zawel¹, J L Dai, P Buckhaults, S Zhou, K W Kinzler, B Vogelstein, S E Kern.

Abstract

Mounting evidence indicates that Smad proteins are required for TGF beta signaling, but the way(s) in which Smad proteins propagate this signal is unclear. We found that two human Smad proteins (Smad3 and Smad4) could specifically recognize an identical 8 bp palindromic sequences (GTCTAGAC). Tandem repeats of this palindrome conferred striking TGF beta responsiveness to a minimal promoter. This responsiveness was abrogated by targeted deletion of the cellular Smad4 gene. These results define a novel biochemical property of Smad proteins that is likely to play a direct role in the biologic responses to TGF beta and related ligands.

Entities: Gene Species

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Year: 1998 PMID： 9660945 DOI： 10.1016/s1097-2765(00)80061-1

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970

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Cited

329 in total

Review 1. Transcriptional control by the TGF-beta/Smad signaling system.

Authors: J Massagué; D Wotton
Journal: EMBO J Date: 2000-04-17 Impact factor: 11.598

2. A distinct nuclear localization signal in the N terminus of Smad 3 determines its ligand-induced nuclear translocation.

Authors: Z Xiao; X Liu; Y I Henis; H F Lodish
Journal: Proc Natl Acad Sci U S A Date: 2000-07-05 Impact factor: 11.205

3. A novel smad nuclear interacting protein, SNIP1, suppresses p300-dependent TGF-beta signal transduction.

Authors: R H Kim; D Wang; M Tsang; J Martin; C Huff; M P de Caestecker; W T Parks; X Meng; R J Lechleider; T Wang; A B Roberts
Journal: Genes Dev Date: 2000-07-01 Impact factor: 11.361

4. Structural basis for the functional difference between Smad2 and Smad3 in FAST-2 (forkhead activin signal transducer-2)-mediated transcription.

Authors: R P Nagarajan; Y Chen
Journal: Biochem J Date: 2000-08-15 Impact factor: 3.857

Human Smad3 and Smad4 are sequence-specific transcription activators.

Review 1. Transcriptional control by the TGF-beta/Smad signaling system.

2. A distinct nuclear localization signal in the N terminus of Smad 3 determines its ligand-induced nuclear translocation.

3. A novel smad nuclear interacting protein, SNIP1, suppresses p300-dependent TGF-beta signal transduction.

4. Structural basis for the functional difference between Smad2 and Smad3 in FAST-2 (forkhead activin signal transducer-2)-mediated transcription.

5. Smad proteins regulate transcriptional induction of the SM22alpha gene by TGF-beta.

6. Inactivation of smad-transforming growth factor beta signaling by Ca(2+)-calmodulin-dependent protein kinase II.

7. Smads bind directly to the Jun family of AP-1 transcription factors.

8. Menin-mediated caspase 8 expression in suppressing multiple endocrine neoplasia type 1.

9. Myostatin signals through a transforming growth factor beta-like signaling pathway to block adipogenesis.

10. G1 cell cycle arrest and apoptosis induction by nuclear Smad4/Dpc4: phenotypes reversed by a tumorigenic mutation.