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Literature DB >> 9660920

Identification of a tRNA-specific nuclear export receptor.

U Kutay¹, G Lipowsky, E Izaurralde, F R Bischoff, P Schwarzmaier, E Hartmann, D Görlich.

Abstract

In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.

Entities: Chemical Gene Species

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Year: 1998 PMID： 9660920 DOI： 10.1016/s1097-2765(00)80036-2

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970

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Cited

169 in total

1. Defects in tRNA processing and nuclear export induce GCN4 translation independently of phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2.

Authors: H Qiu; C Hu; J Anderson; G R Björk; S Sarkar; A K Hopper; A G Hinnebusch
Journal: Mol Cell Biol Date: 2000-04 Impact factor: 4.272

Review 2. Nuclear RNA export pathways.

Authors: B R Cullen
Journal: Mol Cell Biol Date: 2000-06 Impact factor: 4.272

Identification of a tRNA-specific nuclear export receptor.

1. Defects in tRNA processing and nuclear export induce GCN4 translation independently of phosphorylation of the alpha subunit of eukaryotic translation initiation factor 2.

Review 2. Nuclear RNA export pathways.

3. RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro.

4. Coordination of tRNA nuclear export with processing of tRNA.

5. beta-catenin can be transported into the nucleus in a Ran-unassisted manner.

6. The carboxyl terminus of RNA helicase A contains a bidirectional nuclear transport domain.

7. Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes.

8. Selective importation of RNA into isolated mitochondria from Leishmania tarentolae.

Review 9. Transport into and out of the nucleus.

10. Multiple vesiculoviral matrix proteins inhibit both nuclear export and import.