Literature DB >> 965844

Determination of protein in adipose tissue extracts.

H Tornqvist, P Belfrage.   

Abstract

An accurate and sensitive method for determination of protein in adipose tissue extracts based on a scaled-down version of the method of Lowry et al. (1951, J. Biol. Chem. 193, 265-275) is described. Protein is quantitatively precipitated by trichloroacetic acid with a nonionic detergent as a bulk-increasing substance and the precipitate is extracted with an organic solvent. This interference caused by lipids, sulfhydryl reagents, and several other compounds is eliminated. The accuracy of the determination of dilute reference protein solutions (2-20 mug/ml) is demonstrated.

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Year:  1976        PMID： 965844

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  8 in total

1.  Uptake and degradation of iodine-labelled chylomicron remnant particles by monolayers of rat hepatocytes.

Authors:  C H Florén; A Nilsson
Journal:  Biochem J       Date:  1978-09-15       Impact factor: 3.857

2.  Characteristics of Sucrose Transport and Sucrose-Induced H+ Transport on the Tonoplast of Red Beet (Beta vulgaris L.) Storage Tissue.

Authors:  H. P. Getz; M. Klein
Journal:  Plant Physiol       Date:  1995-02       Impact factor: 8.340

3.  The activities of lipoprotein lipase and of enzymes involved in triacylglycerol synthesis in rat adipose tissue. Effects of starvation, dietary modification and of corticotropin injection.

Authors:  N Lawson; A D Pollard; R J Jennings; M I Gurr; D N Brindley
Journal:  Biochem J       Date:  1981-11-15       Impact factor: 3.857

4.  Properties of rat erythrocyte membrane cytoskeletal structures produced by digitonin extraction: digitonin-insoluble beta-adrenergic receptor, adenylate cyclase, and cholera toxin substrate.

Authors:  H LeVine; N E Sahyoun; P Cuatrecasas
Journal:  J Membr Biol       Date:  1982       Impact factor: 1.843

5.  Specific phospholipids are required to reconstitute adenylate cyclase solubilized from rat brain.

Authors:  G M Hebdon; H LeVine; N E Sahyoun; C J Schmitges; P Cuatrecasas
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

6.  Generation of a stable, posttranslationally modified microtubule array is an early event in myogenic differentiation.

Authors:  G G Gundersen; S Khawaja; J C Bulinski
Journal:  J Cell Biol       Date:  1989-11       Impact factor: 10.539

7.  Sperm surface proteins persist after fertilization.

Authors:  G G Gundersen; B M Shapiro
Journal:  J Cell Biol       Date:  1984-10       Impact factor: 10.539

8.  Postpolymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules.

Authors:  G G Gundersen; S Khawaja; J C Bulinski
Journal:  J Cell Biol       Date:  1987-07       Impact factor: 10.539
  8 in total

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