Interactions of pancreatic lipase with bile salts and dodecyl sulfate.

Sodium dodecyl sulfate (SDS) binds to pancreatic lipase in a cooperative manner up to about 200 moles/mole of protein. This binding in a rapid and irreversible inactivation of lipase. Bile salts under certain conditions prevent SDS inactivation of lipase when both detergents are present together. Under these conditions bile salts also prevent the binding of SDS to lipase. This effect is parallel to and probably mediated by a decrease in the SDS monomer concentration as the result of the formation of mixed bile salt-SDS micelles.