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Literature DB >> 9652137

Hybrid enzymes: manipulating enzyme design.

Abstract

Hybrid enzymes are engineered to contain elements of two or more enzymes. Hybrid-enzyme approaches, by taking advantage of the vast array of enzymatic properties that nature has evolved, as well as the strategies that nature has used to evolve them, are becoming an increasingly important avenue for obtaining novel enzymes with desired activities and properties.

Mesh：

Substances：
Enzymes

Year: 1998 PMID： 9652137 DOI： 10.1016/s0167-7799(98)01204-9

Source DB: PubMed Journal: Trends Biotechnol ISSN： 0167-7799 Impact factor: 19.536

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Cited

17 in total

1. Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formation.

Authors: Giedrius Vilkaitis; Arvydas Lubys; Egle Merkiene; Albertas Timinskas; Arvydas Janulaitis; Saulius Klimasauskas
Journal: Nucleic Acids Res Date: 2002-04-01 Impact factor: 16.971

2. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2.

Authors: V Abécassis; D Pompon; G Truan
Journal: Nucleic Acids Res Date: 2000-10-15 Impact factor: 16.971

3. A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.

Authors: Jean-Charles Epinat; Sylvain Arnould; Patrick Chames; Pascal Rochaix; Dominique Desfontaines; Clémence Puzin; Amélie Patin; Alexandre Zanghellini; Frédéric Pâques; Emmanuel Lacroix
Journal: Nucleic Acids Res Date: 2003-06-01 Impact factor: 16.971

4. Helix-hairpin-helix motifs confer salt resistance and processivity on chimeric DNA polymerases.

Authors: Andrey R Pavlov; Galina I Belova; Sergei A Kozyavkin; Alexei I Slesarev
Journal: Proc Natl Acad Sci U S A Date: 2002-10-04 Impact factor: 11.205

5. Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance.

Authors: Lucas F Ribeiro; Gilvan P Furtado; Marcos R Lourenzoni; Antonio J Costa-Filho; Camila R Santos; Simone C Peixoto Nogueira; Jorge A Betini; Maria de Lourdes T M Polizeli; Mario T Murakami; Richard J Ward
Journal: J Biol Chem Date: 2011-10-17 Impact factor: 5.157

6. Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains.

Authors: Sonata Jurenaite-Urbanaviciene; Jurgita Serksnaite; Edita Kriukiene; Jolanta Giedriene; Ceslovas Venclovas; Arvydas Lubys
Journal: Proc Natl Acad Sci U S A Date: 2007-06-06 Impact factor: 11.205

Hybrid enzymes: manipulating enzyme design.

1. Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formation.

2. High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2.

3. A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.

4. Helix-hairpin-helix motifs confer salt resistance and processivity on chimeric DNA polymerases.

5. Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance.

6. Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains.

7. A chimeric Cre recombinase with regulated directionality.

Review 8. Bifunctional xylanases and their potential use in biotechnology.

9. Rational design of a scytalone dehydratase-like enzyme using a structurally homologous protein scaffold.

10. Engineering isoflavone metabolism with an artificial bifunctional enzyme.