Literature DB >> 9650967

Molecular diagnosis of human rhinovirus infections: comparison with virus isolation.

T Hyypiä1, T Puhakka, O Ruuskanen, M Mäkelä, A Arola, P Arstila.   

Abstract

To compare the sensitivity and specificity of RT-PCR with that of virus isolation in the detection of human rhinoviruses, we tested nasopharyngeal aspirates from 200 patients on the 1st and 7th days after the onset of the common cold. An assay utilizing a short amplicon in the conserved 5' noncoding region was found highly sensitive. Of 192 positive samples altogether, 65 were found positive by RT-PCR only, 6 were positive by isolation exclusively, and 121 gave positive results in both tests.

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Year:  1998        PMID： 9650967      PMCID： PMC104983     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Polymerase chain reaction for human picornaviruses.

Authors:  T Hyypiä; P Auvinen; M Maaronen
Journal:  J Gen Virol       Date:  1989-12       Impact factor: 3.891

2.  Computed tomographic study of the common cold.

Authors:  J M Gwaltney; C D Phillips; R D Miller; D K Riker
Journal:  N Engl J Med       Date:  1994-01-06       Impact factor: 91.245

3.  Detection of enteroviruses by spot hybridization.

Authors:  T Hyypiä; P Stålhandske; R Vainionpää; U Pettersson
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

4.  Detection and differentiation of picornaviruses in clinical samples following genomic amplification.

Authors:  D M Olive; S Al-Mufti; W Al-Mulla; M A Khan; A Pasca; G Stanway; W Al-Nakib
Journal:  J Gen Virol       Date:  1990-09       Impact factor: 3.891

5.  Rhinovirus in otitis media with effusion.

Authors:  M Arola; T Ziegler; H Puhakka; O P Lehtonen; O Ruuskanen
Journal:  Ann Otol Rhinol Laryngol       Date:  1990-06       Impact factor: 1.547

6.  Amplification of rhinovirus specific nucleic acids from clinical samples using the polymerase chain reaction.

Authors:  R E Gama; P R Horsnell; P J Hughes; C North; C B Bruce; W al-Nakib; G Stanway
Journal:  J Med Virol       Date:  1989-06       Impact factor: 2.327

7.  Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms.

Authors:  S L Johnston; G Sanderson; P K Pattemore; S Smith; P G Bardin; C B Bruce; P R Lambden; D A Tyrrell; S T Holgate
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948

8.  Detection of human rhinovirus RNA in nasal washings by PCR.

Authors:  E Arruda; F G Hayden
Journal:  Mol Cell Probes       Date:  1993-10       Impact factor: 2.365

9.  Respiratory viruses and exacerbations of asthma in adults.

Authors:  K G Nicholson; J Kent; D C Ireland
Journal:  BMJ       Date:  1993-10-16

10.  Viruses and bacteria in the etiology of the common cold.

Authors:  M J Mäkelä; T Puhakka; O Ruuskanen; M Leinonen; P Saikku; M Kimpimäki; S Blomqvist; T Hyypiä; P Arstila
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948
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  29 in total

1.  Detection of rhinoviruses by tissue culture and two independent amplification techniques, nucleic acid sequence-based amplification and reverse transcription-PCR, in children with acute respiratory infections during a winter season.

Authors:  K Loens; H Goossens; C de Laat; H Foolen; P Oudshoorn; S Pattyn; P Sillekens; M Ieven
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

2.  Improved detection of rhinoviruses in clinical samples by using a newly developed nested reverse transcription-PCR assay.

Authors:  A C Andeweg; T M Bestebroer; M Huybreghs; T G Kimman; J C de Jong
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

3.  Role of picornaviruses in flu-like illnesses of adults enrolled in an oseltamivir treatment study who had no evidence of influenza virus infection.

Authors:  Guy Boivin; Albert D Osterhaus; Annie Gaudreau; Helen C Jackson; Jan Groen; Penelope Ward
Journal:  J Clin Microbiol       Date:  2002-02       Impact factor: 5.948

4.  Rapid detection of human rhinoviruses in nasopharyngeal aspirates by a microwell reverse transcription-PCR-hybridization assay.

Authors:  S Blomqvist; A Skyttä; M Roivainen; T Hovi
Journal:  J Clin Microbiol       Date:  1999-09       Impact factor: 5.948

5.  Simultaneous detection and differentiation of human rhino- and enteroviruses in clinical specimens by real-time PCR with locked nucleic Acid probes.

Authors:  Riikka Osterback; Tuire Tevaluoto; Tiina Ylinen; Ville Peltola; Petri Susi; Timo Hyypiä; Matti Waris
Journal:  J Clin Microbiol       Date:  2013-09-18       Impact factor: 5.948

Review 6.  Detection of respiratory viruses by molecular methods.

Authors:  James B Mahony
Journal:  Clin Microbiol Rev       Date:  2008-10       Impact factor: 26.132

7.  Amplicon sequencing and improved detection of human rhinovirus in respiratory samples.

Authors:  Christelle Deffernez; Werner Wunderli; Yves Thomas; Sabine Yerly; Luc Perrin; Laurent Kaiser
Journal:  J Clin Microbiol       Date:  2004-07       Impact factor: 5.948

8.  Rhinovirus-associated wheeze during infancy and asthma development.

Authors:  Tuomas Jartti; James E Gern
Journal:  Curr Respir Med Rev       Date:  2011-06-01

9.  Epidemiological, molecular, and clinical features of enterovirus respiratory infections in French children between 1999 and 2005.

Authors:  Jérôme Jacques; Hélène Moret; Delphine Minette; Nicolas Lévêque; Nicolas Jovenin; Gaetan Deslée; François Lebargy; Jacques Motte; Laurent Andréoletti
Journal:  J Clin Microbiol       Date:  2007-11-14       Impact factor: 5.948

Review 10.  Importance of respiratory viruses in acute otitis media.

Authors:  Terho Heikkinen; Tasnee Chonmaitree
Journal:  Clin Microbiol Rev       Date:  2003-04       Impact factor: 26.132
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