Literature DB >> 9647849

Specific detection of Legionella pneumophila: construction of a new 16S rRNA-targeted oligonucleotide probe.

D Grimm1, H Merkert, W Ludwig, K H Schleifer, J Hacker, B C Brand.   

Abstract

Based on comparative sequence analysis, we have designed an oligonucleotide probe complementary to a region of 16S rRNA of Legionella pneumophila which allows the differentiation of L. pneumophila from other Legionella species without cultivation. The specificity of the new probe, LEGPNE1, was tested by in situ hybridization to a total of four serogroups of six strains of L. pneumophila, five different Legionella spp. and three nonlegionella species as reference strains. Furthermore, L. pneumophila cells could be easily distinguished from Legionella micdadei and Pseudomonas aeruginosa cells by using in situ hybridization with probes LEGPNE1, LEG705, and EUB338 after infection of the protozoan Acanthamoeba castellanii.

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Year:  1998        PMID： 9647849      PMCID： PMC106445     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

1.  Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

Authors:  R I Amann; B J Binder; R J Olson; S W Chisholm; R Devereux; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

2.  Immunogold and fluorescein immunolabelling of Legionella pneumophila within an aquatic biofilm visualized by using episcopic differential interference contrast microscopy.

Authors:  J Rogers; C W Keevil
Journal:  Appl Environ Microbiol       Date:  1992-07       Impact factor: 4.792

3.  Identification in situ and phylogeny of uncultured bacterial endosymbionts.

Authors:  R Amann; N Springer; W Ludwig; H D Görtz; K H Schleifer
Journal:  Nature       Date:  1991-05-09       Impact factor: 49.962

4.  Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge.

Authors:  M Wagner; R Erhart; W Manz; R Amann; H Lemmer; D Wedi; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1994-03       Impact factor: 4.792

5.  A quantitative model of intracellular growth of Legionella pneumophila in Acanthamoeba castellanii.

Authors:  J F Moffat; L S Tompkins
Journal:  Infect Immun       Date:  1992-01       Impact factor: 3.441

6.  Problems associated with identification of Legionella species from the environment and isolation of six possible new species.

Authors:  I J Wilkinson; N Sangster; R M Ratcliff; P A Mugg; D E Davos; J A Lanser
Journal:  Appl Environ Microbiol       Date:  1990-03       Impact factor: 4.792

7.  Detection of Legionella species in sewage and ocean water by polymerase chain reaction, direct fluorescent-antibody, and plate culture methods.

Authors:  C J Palmer; Y L Tsai; C Paszko-Kolva; C Mayer; L R Sangermano
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

8.  Spatial distribution of Escherichia coli in the mouse large intestine inferred from rRNA in situ hybridization.

Authors:  L K Poulsen; F Lan; C S Kristensen; P Hobolth; S Molin; K A Krogfelt
Journal:  Infect Immun       Date:  1994-11       Impact factor: 3.441

9.  Distribution, expression, and long-range mapping of legiolysin gene (lly)-specific DNA sequences in legionellae.

Authors:  L Bender; M Ott; A Debes; U Rdest; J Heesemann; J Hacker
Journal:  Infect Immun       Date:  1991-09       Impact factor: 3.441

10.  In situ identification of Legionellaceae using 16S rRNA-targeted oligonucleotide probes and confocal laser scanning microscopy.

Authors:  W Manz; R Amann; R Szewzyk; U Szewzyk; T A Stenström; P Hutzler; K H Schleifer
Journal:  Microbiology       Date:  1995-01       Impact factor: 2.777
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  14 in total

1.  Detection and differentiation of chlamydiae by fluorescence in situ hybridization.

Authors:  Sven Poppert; Andreas Essig; Reinhard Marre; Michael Wagner; Matthias Horn
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792

2.  Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of the dotA gene.

Authors:  M A Yáñez; C Carrasco-Serrano; V M Barberá; V Catalán
Journal:  Appl Environ Microbiol       Date:  2005-07       Impact factor: 4.792

3.  Impact of non-Legionella bacteria on the uptake and intracellular replication of Legionella pneumophila in Acanthamoeba castellanii and Naegleria lovaniensis.

Authors:  P Declerck; J Behets; Y Delaedt; A Margineanu; E Lammertyn; F Ollevier
Journal:  Microb Ecol       Date:  2005-12-15       Impact factor: 4.552

4.  Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms.

Authors:  Sandra A Wilks; C William Keevil
Journal:  Appl Environ Microbiol       Date:  2006-08       Impact factor: 4.792

5.  Flagellum of Legionella pneumophila positively affects the early phase of infection of eukaryotic host cells.

Authors:  C Dietrich; K Heuner; B C Brand; J Hacker; M Steinert
Journal:  Infect Immun       Date:  2001-04       Impact factor: 3.441

6.  Detection of Staphylococcus aureus and Staphylococcus epidermidis in clinical samples by 16S rRNA-directed in situ hybridization.

Authors:  V Krimmer; H Merkert; C von Eiff; M Frosch; J Eulert; J F Löhr; J Hacker; W Ziebuhr
Journal:  J Clin Microbiol       Date:  1999-08       Impact factor: 5.948

7.  Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture.

Authors:  R T Hayden; J R Uhl; X Qian; M K Hopkins; M C Aubry; A H Limper; R V Lloyd; F R Cockerill
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

8.  Intracellular proliferation of Legionella pneumophila in Hartmannella vermiformis in aquatic biofilms grown on plasticized polyvinyl chloride.

Authors:  Melanie W Kuiper; Bart A Wullings; Antoon D L Akkermans; Rijkelt R Beumer; Dick van der Kooij
Journal:  Appl Environ Microbiol       Date:  2004-11       Impact factor: 4.792

9.  Species-specific oligonucleotides for enumeration of Pseudomonas putida F1, Burkholderia sp. strain JS150, and Bacillus subtilis ATCC 7003 in biodegradation experiments.

Authors:  N M DuTeau; J D Rogers; C T Bartholomay; K F Reardon
Journal:  Appl Environ Microbiol       Date:  1998-12       Impact factor: 4.792

10.  Identification of Legionella spp. in Environmental Water Samples by ScanVIT-Legionella™ Method in Spain.

Authors:  Cristina Gruas; Isidro Alvarez; Carlos Lara; Cristina Belén García; Demetris Savva; M Victoria Arruga
Journal:  Indian J Microbiol       Date:  2013-02-03       Impact factor: 2.461
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