Literature DB >> 9647791

Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8).

T Nishida1, T Nakanishi, T Shimo, M Asano, T Hattori, T Tamatani, K Tezuka, M Takigawa.   

Abstract

The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8. The binding of 125I-labeled recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Kd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of 125I-CTGF-receptor complex with an apparent molecular weight of 280 kDa. The 125I-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF. In addition, the 125I-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells.

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Year:  1998        PMID： 9647791     DOI： 10.1006/bbrc.1998.8895

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  20 in total

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Authors:  B Perbal
Journal:  Mol Pathol       Date:  2001-04

Review 2.  The role of connective tissue growth factor (CTGF/CCN2) in skeletogenesis.

Authors:  John A Arnott; Alex G Lambi; Christina Mundy; Honey Hendesi; Robin A Pixley; Thomas A Owen; Fayez F Safadi; Steven N Popoff
Journal:  Crit Rev Eukaryot Gene Expr       Date:  2011       Impact factor: 1.807

3.  CCN family 2/connective tissue growth factor (CCN2/CTGF) promotes osteoclastogenesis via induction of and interaction with dendritic cell-specific transmembrane protein (DC-STAMP).

Authors:  Takashi Nishida; Kenji Emura; Satoshi Kubota; Karen M Lyons; Masaharu Takigawa
Journal:  J Bone Miner Res       Date:  2011-02       Impact factor: 6.741

4.  CCN family protein 2 (CCN2) promotes the early differentiation, but inhibits the terminal differentiation of skeletal myoblasts.

Authors:  Takashi Nishida; Satoshi Kubota; Eriko Aoyama; Danilo Janune; Karen M Lyons; Masaharu Takigawa
Journal:  J Biochem       Date:  2014-09-26       Impact factor: 3.387

Review 5.  Cell surface receptors for CCN proteins.

Authors:  Lester F Lau
Journal:  J Cell Commun Signal       Date:  2016-04-20       Impact factor: 5.782

6.  Connective tissue growth factor is required for normal follicle development and ovulation.

Authors:  Takashi Nagashima; Jaeyeon Kim; Qinglei Li; John P Lydon; Francesco J DeMayo; Karen M Lyons; Martin M Matzuk
Journal:  Mol Endocrinol       Date:  2011-08-25

7.  Steroidal regulation of connective tissue growth factor (CCN2; CTGF) synthesis in the mouse uterus.

Authors:  M A Rageh; E E Moussad; A K Wilson; D R Brigstock
Journal:  Mol Pathol       Date:  2001-10

8.  Uptake and intracellular transport of the connective tissue growth factor: a potential mode of action.

Authors:  N A Wahab; H Brinkman; R M Mason
Journal:  Biochem J       Date:  2001-10-01       Impact factor: 3.857

9.  The Twisted gastrulation family of proteins, together with the IGFBP and CCN families, comprise the TIC superfamily of cysteine rich secreted factors.

Authors:  P Vilmos; K Gaudenz; Z Hegedus; J L Marsh
Journal:  Mol Pathol       Date:  2001-10

10.  CCN family 2/connective tissue growth factor modulates BMP signalling as a signal conductor, which action regulates the proliferation and differentiation of chondrocytes.

Authors:  Azusa Maeda; Takashi Nishida; Eriko Aoyama; Satoshi Kubota; Karen M Lyons; Takuo Kuboki; Masaharu Takigawa
Journal:  J Biochem       Date:  2008-11-27       Impact factor: 3.387
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