S K Lam1, P L Devine. 1. WHO Collaborating Centre for Arbovirus Reference and Research (Dengue and Dengue Hemorrhagic Fever), Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. lamsk@medicine.med.um.edu.my
Abstract
BACKGROUND: Rapid diagnosis of dengue infection is essential to patient management and disease control. The development of a rapid (5 min) immunochromatographic test and a 2 h commercial capture enzyme linked immunosorbent assay (ELISA) for anti-dengue IgM and IgG antibodies may lead to more rapid and accurate testing in peripheral health settings and diagnostic laboratories. OBJECTIVES: Evaluate two new commercial tests for dengue serology (Dengue Rapid test and Dengue Duo ELISA; PanBio, Brisbane, Australia). STUDY DESIGN: The sensitivity and specificity of the tests were compared with in-house dengue IgM ELISA and hemagglutination-inhibition (HAI) assays using known positive and negative dengue specimens, as well as specimens from non-dengue cases. RESULTS: Both assays showed excellent sensitivity in the diagnosis of both primary and secondary dengue infection (100%). In both assays, IgG levels showed excellent correlation with the hemagglutination-inhibition (HAI) assay, and these could be used to distinguish between primary and secondary dengue infections (92 and 97% of patients correctly classified in the rapid test and Duo ELISA, respectively). Specificity in both assays was 89% when sera from patients, with no apparent dengue infection, typhoid, leptospirosis and malaria, were tested. CONCLUSIONS: These tests should be a useful aid in confirming the clinical diagnosis of dengue infection. The rapid test will be particularly valuable in peripheral health settings, while the ELISA has a place in central testing laboratories.
BACKGROUND: Rapid diagnosis of dengue infection is essential to patient management and disease control. The development of a rapid (5 min) immunochromatographic test and a 2 h commercial capture enzyme linked immunosorbent assay (ELISA) for anti-dengue IgM and IgG antibodies may lead to more rapid and accurate testing in peripheral health settings and diagnostic laboratories. OBJECTIVES: Evaluate two new commercial tests for dengue serology (Dengue Rapid test and Dengue Duo ELISA; PanBio, Brisbane, Australia). STUDY DESIGN: The sensitivity and specificity of the tests were compared with in-house dengue IgM ELISA and hemagglutination-inhibition (HAI) assays using known positive and negative dengue specimens, as well as specimens from non-dengue cases. RESULTS: Both assays showed excellent sensitivity in the diagnosis of both primary and secondary dengue infection (100%). In both assays, IgG levels showed excellent correlation with the hemagglutination-inhibition (HAI) assay, and these could be used to distinguish between primary and secondary dengue infections (92 and 97% of patients correctly classified in the rapid test and Duo ELISA, respectively). Specificity in both assays was 89% when sera from patients, with no apparent dengue infection, typhoid, leptospirosis and malaria, were tested. CONCLUSIONS: These tests should be a useful aid in confirming the clinical diagnosis of dengue infection. The rapid test will be particularly valuable in peripheral health settings, while the ELISA has a place in central testing laboratories.
Authors: A J Cuzzubbo; T P Endy; A Nisalak; S Kalayanarooj; D W Vaughn; S A Ogata; D E Clements; P L Devine Journal: Clin Diagn Lab Immunol Date: 2001-11
Authors: Subhamoy Pal; Allison L Dauner; Andrea Valks; Brett M Forshey; Kanya C Long; Butsaya Thaisomboonsuk; Gloria Sierra; Victor Picos; Sara Talmage; Amy C Morrison; Eric S Halsey; Guillermo Comach; Chadwick Yasuda; Michael Loeffelholz; Richard G Jarman; Stefan Fernandez; Ung Sam An; Tadeusz J Kochel; Louis E Jasper; Shuenn-Jue L Wu Journal: J Clin Microbiol Date: 2015-01-14 Impact factor: 5.948