Literature DB >> 9645237

Detection of Japanese encephalitis virus by reverse transcription/polymerase chain reaction.

S Paranjpe1, K Banerjee.   

Abstract

A simple and rapid reverse transcription/polymerase chain reaction (RT-PCR) assay for detection of Japanese encephalitis virus (JEV) envelope (E) gene sequences in various biological samples is described. The assay successfully amplified JEV E gene sequences from infected cell cultures, Aedes aegypti larvae, mosquitoes and mouse blood. The sensitivity of the assay was currently 1 ng of JEV RNA and could be increased up to 1 pg on the background of 1 microgram of cellular RNA by biotinylation of the PCR product, Southern blot analysis and streptavidin/alkaline phosphatase detection.

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Year:  1998        PMID： 9645237

Source DB:  PubMed          Journal:  Acta Virol        ISSN： 0001-723X            Impact factor:   1.162


  6 in total

Review 1.  Application of nucleic acid amplification in clinical microbiology.

Authors:  G Lisby
Journal:  Mol Biotechnol       Date:  1999-08       Impact factor: 2.695

2.  Comparison of reverse transcription loop-mediated isothermal amplification, conventional PCR and real-time PCR assays for Japanese encephalitis virus.

Authors:  Zhiyong Chen; Yuxue Liao; Xuemei Ke; Jie Zhou; Yixiong Chen; LuLu Gao; Qing Chen; Shouyi Yu
Journal:  Mol Biol Rep       Date:  2010-11-30       Impact factor: 2.316

Review 3.  Japanese viral encephalitis.

Authors:  S V Tiroumourougane; P Raghava; S Srinivasan
Journal:  Postgrad Med J       Date:  2002-04       Impact factor: 2.401

4.  Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine.

Authors:  Dong Kun Yang; Byoung Han Kim; Seong In Lim; Jun Hun Kwon; Kyung Woo Lee; Cheong Up Choi; Chang Hee Kweon
Journal:  J Vet Sci       Date:  2006-09       Impact factor: 1.672

5.  Use of a Multiplex RT-PCR Assay for Simultaneous Detection of the North American Genotype Porcine Reproductive and Respiratory Syndrome Virus, Swine Influenza Virus and Japanese Encephalitis Virus.

Authors:  Hong-Ying Chen; Zhan-Yong Wei; Hong-Ying Zhang; Xiao-Li Lü; Lan-Lan Zheng; Bao-An Cui; Jinpeng Liu; Qian-Lei Zhu; Zi-Xin Wang
Journal:        Date:  2010-07-17

6.  Sensitive and specific detection of strains of Japanese encephalitis virus using a one-step TaqMan RT-PCR technique.

Authors:  Jau-Ling Huang; Hui-Tsu Lin; Yu-Ming Wang; Ming-Hui Weng; Da-Der Ji; Ming-Der Kuo; Huan-Wun Liu; Chang-Shen Lin
Journal:  J Med Virol       Date:  2004-12       Impact factor: 2.327
  6 in total

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