Literature DB >> 9642207

Mutants of Citrobacter freundii that transport and utilize melibiose.

N Okazaki1, X J Xu, T Shimamoto, M Kuroda, T H Wilson, T Tsuchiya.   

Abstract

We have isolated mutants of Citrobacter freundii that can grow on melibiose. Inducible alpha-galactosidase activity and melibiose transport activity were detected in the mutant cells but not in the wild-type cells. We detected a DNA region which hybridized with melB (the gene for the melibiose transporter) DNA of Escherichia coli in the chromosomal DNA of wild-type C. freundii. Protons, but not sodium ions, were found to be the coupling cations for melibiose (and methyl-beta-D-thiogalactoside) transport in the mutant cells.

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Year:  1998        PMID： 9642207      PMCID： PMC107309     

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  17 in total

1.  Transduction of linked genetic characters of the host by bacteriophage P1.

Authors:  E S LENNOX
Journal:  Virology       Date:  1955-07       Impact factor: 3.616

2.  Cloning and sequencing of the melB gene encoding the melibiose permease of Salmonella typhimurium LT2.

Authors:  K Mizushima; S Awakihara; M Kuroda; T Ishikawa; M Tsuda; T Tsuchiya
Journal:  Mol Gen Genet       Date:  1992-07

3.  Role of Na+ and Li+ in thiomethylgalactoside transport by the melibiose transport system of Escherichia coli.

Authors:  J Lopilato; T Tsuchiya; T H Wilson
Journal:  J Bacteriol       Date:  1978-04       Impact factor: 3.490

4.  Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli.

Authors:  H Yazyu; S Shiota-Niiya; T Shimamoto; H Kanazawa; M Futai; T Tsuchiya
Journal:  J Biol Chem       Date:  1984-04-10       Impact factor: 5.157

5.  Physical and genetic characterization of the melibiose operon and identification of the gene products in Escherichia coli.

Authors:  M Hanatani; H Yazyu; S Shiota-Niiya; Y Moriyama; H Kanazawa; M Futai; T Tsuchiya
Journal:  J Biol Chem       Date:  1984-02-10       Impact factor: 5.157

6.  Alteration in cation specificity of the melibiose transport carrier of Escherichia coli due to replacement of proline 122 with serine.

Authors:  H Yazyu; S Shiota; M Futai; T Tsuchiya
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

7.  Effect of lithium ion on melibiose transport in Escherichia coli.

Authors:  T Tsuchiya; J Lopilato; T H Wilson
Journal:  J Membr Biol       Date:  1978-07-21       Impact factor: 1.843

8.  Primary structure and characteristics of the melibiose carrier of Klebsiella pneumoniae.

Authors:  H Hama; T H Wilson
Journal:  J Biol Chem       Date:  1992-09-15       Impact factor: 5.157

9.  Replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol.

Authors:  S Tanaka; S A Lerner; E C Lin
Journal:  J Bacteriol       Date:  1967-02       Impact factor: 3.490

10.  Cation coupling to melibiose transport in Salmonella typhimurium.

Authors:  S Niiya; Y Moriyama; M Futai; T Tsuchiya
Journal:  J Bacteriol       Date:  1980-10       Impact factor: 3.490
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