Literature DB >> 9639888

Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods.

R M Wartell1, S Hosseini, S Powell, J Zhu.   

Abstract

Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting point mutations, and evaluating the effects of single site defects are also reported.

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Year:  1998        PMID： 9639888     DOI： 10.1016/s0021-9673(98)00149-6

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  6 in total

1.  Solution-based scanning for single-base alterations using a double-stranded DNA binding dye and fluorescence-melting profiles.

Authors:  K S Elenitoba-Johnson; S D Bohling
Journal:  Am J Pathol       Date:  2001-09       Impact factor: 4.307

2.  Commonly conserved genetic fragments revealed by genome profiling can serve as tracers of evolution.

Authors:  Mohammed Naimuddin; Takayuki Kurazono; Koichi Nishigaki
Journal:  Nucleic Acids Res       Date:  2002-05-15       Impact factor: 16.971

3.  Change of the protein p53 electrochemical signal according to its structural form - quick and sensitive distinguishing of native, denatured, and aggregated form of the "guardian of the genome".

Authors:  David Potesil; Radka Mikelova; Vojtech Adam; Rene Kizek; Richard Prusa
Journal:  Protein J       Date:  2006-01       Impact factor: 4.000

4.  Genome profiling (GP) method based classification of insects: congruence with that of classical phenotype-based one.

Authors:  Shamim Ahmed; Manabu Komori; Sachika Tsuji-Ueno; Miho Suzuki; Akinori Kosaku; Kiyoshi Miyamoto; Koichi Nishigaki
Journal:  PLoS One       Date:  2011-08-31       Impact factor: 3.240

5.  A database for the provisional identification of species using only genotypes: web-based genome profiling.

Authors:  Takehiro Watanabe; Ayumu Saito; Yusuke Takeuchi; Mohammed Naimuddin; Koichi Nishigaki
Journal:  Genome Biol       Date:  2002-01-28       Impact factor: 13.583

6.  Heat-transfer-based detection of SNPs in the PAH gene of PKU patients.

Authors:  Natalie Vanden Bon; Bart van Grinsven; Mohammed Sharif Murib; Weng Siang Yeap; Ken Haenen; Ward De Ceuninck; Patrick Wagner; Marcel Ameloot; Veronique Vermeeren; Luc Michiels
Journal:  Int J Nanomedicine       Date:  2014-03-27
  6 in total

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