Literature DB >> 9636307

Fusarium graminearum A 3/5 as a novel host for heterologous protein production.

J C Royer1, D L Moyer, S G Reiwitch, M S Madden, E B Jensen, S H Brown, C C Yonker, J A Johnston, E J Golightly, W T Yoder.   

Abstract

We describe a novel fungal expression system which utilizes the Quorn myco-protein fungus Fusarium graminearum A 3/5. A transformation system was developed for F. graminearum and was used to introduce the coding and regulatory regions of a trypsin gene from Fusarium oxysporum. The protein was efficiently expressed, processed and secreted by the recombinant host strain. In addition, the promoter and terminator of the F. oxysporum trypsin gene have been successfully utilized to drive the expression of a cellulase gene from Scytalidium thermophilum and a lipase gene from Thermomyces lanuginosus in F. graminearum.

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Year:  1995        PMID： 9636307     DOI： 10.1038/nbt1295-1479

Source DB:  PubMed          Journal:  Biotechnology (N Y)        ISSN： 0733-222X


  9 in total

1.  Purification, characterization, and heterologous expression in Fusarium venenatum of a novel serine carboxypeptidase from Aspergillus oryzae.

Authors:  A M Blinkovsky; T Byun; K M Brown; E J Golightly
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

2.  Pathogen Trojan Horse Delivers Bioactive Host Protein to Alter Maize Anther Cell Behavior in Situ.

Authors:  Karina van der Linde; Ljudmilla Timofejeva; Rachel L Egger; Birger Ilau; Reza Hammond; Chong Teng; Blake C Meyers; Gunther Doehlemann; Virginia Walbot
Journal:  Plant Cell       Date:  2018-02-15       Impact factor: 11.277

3.  Molecular characterization and expression of a phytase gene from the thermophilic fungus Thermomyces lanuginosus.

Authors:  R M Berka; M W Rey; K M Brown; T Byun; A V Klotz
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

4.  Xyr1 regulates xylanase but not cellulase formation in the head blight fungus Fusarium graminearum.

Authors:  Kurt Brunner; Anton M Lichtenauer; Klaus Kratochwill; Marizela Delic; Robert L Mach
Journal:  Curr Genet       Date:  2007-10-09       Impact factor: 3.886

5.  Mating, conidiation and pathogenicity of Fusarium graminearum, the main causal agent of the head-blight disease of wheat, are regulated by the MAP kinase gpmk1.

Authors:  Nicole J Jenczmionka; Frank J Maier; Anke P Lösch; Wilhelm Schäfer
Journal:  Curr Genet       Date:  2003-03-07       Impact factor: 3.886

6.  Inter-genome comparison of the Quorn fungus Fusarium venenatum and the closely related plant infecting pathogen Fusarium graminearum.

Authors:  Robert King; Neil Andrew Brown; Martin Urban; Kim E Hammond-Kosack
Journal:  BMC Genomics       Date:  2018-04-19       Impact factor: 3.969

7.  Functional Characterization of Calcineurin-Responsive Transcription Factors Fg01341 and Fg01350 in Fusarium graminearum.

Authors:  Xiangxiang Zhang; Shulin Cao; Wei Li; Haiyan Sun; Yuanyu Deng; Aixiang Zhang; Huaigu Chen
Journal:  Front Microbiol       Date:  2020-11-26       Impact factor: 5.640

8.  Establishment of High-Efficiency Screening System for Gene Deletion in Fusarium venenatum TB01.

Authors:  Sheng Tong; Kexin An; Wenyuan Zhou; Wuxi Chen; Yuanxia Sun; Qinhong Wang; Demao Li
Journal:  J Fungi (Basel)       Date:  2022-02-10

9.  Characterization of erythrose reductases from filamentous fungi.

Authors:  Birgit Jovanović; Robert L Mach; Astrid R Mach-Aigner
Journal:  AMB Express       Date:  2013-08-08       Impact factor: 3.298
  9 in total

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