| Literature DB >> 9634789 |
L Sági1, B Panis, S Remy, H Schoofs, K De Smet, R Swennen, B P Cammue.
Abstract
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the beta-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome.Entities:
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Year: 1995 PMID： 9634789 DOI： 10.1038/nbt0595-481
Source DB: PubMed Journal: Biotechnology (N Y) ISSN： 0733-222X