| Literature DB >> 9631066 |
R D Hall1, T Riksen-Bruinsma, G J Weyens, I J Rosquin, P N Denys, I J Evans, J E Lathouwers, M P Lefèbvre, J M Dunwell, A van Tunen, F A Krens.
Abstract
An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.). A polyethylene glycol-mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.Entities:
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Year: 1996 PMID： 9631066 DOI： 10.1038/nbt0996-1133
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908