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Literature DB >> 962884

Radioimmunological identification of tropoelastin.

P Christner, M Dixon, A Cywinski, J Rosenbloom.

Abstract

Antiserum was prepared in sheep against insoluble elastin isolated from embryonic-chick aortae. In an indirect immunoprecipitation test, the antiserum reacted quantitatively with small amounts of radioactively labelled purified tropoelastin prepared from embryonic-chick aortae. The antiserum did not cross-react with chick procollagen, and the antiserum uas used to identify radioactively labelled tropoelastin secreted by chick aorta cells in suspension culture.

Entities: Gene Species

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Year: 1976 PMID： 962884 PMCID： PMC1163887 DOI： 10.1042/bj1570525

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

15 in total

1. Identification of a soluble intermediate during synthesis of elastin by embryonic chick aortae.

Authors: L Murphy; M Harsch; T Mori; J Rosenbloom
Journal: FEBS Lett Date: 1972-03-15 Impact factor: 4.124

2. Biosynthesis and secretion of tropoelastin by chick aorta cells.

Authors: J Rosenbloom; A Cywinski
Journal: Biochem Biophys Res Commun Date: 1976-04-05 Impact factor: 3.575

3. Properties of chick tropoelastin.

Authors: R B Rucker; W Goettlich-Riemann; K Tom
Journal: Biochim Biophys Acta Date: 1973-07-12

4. Isolation and amino acid sequences of tropoelastin peptides.

Authors: J A Foster; E Bruenger; W R Gray; L B Sandberg
Journal: J Biol Chem Date: 1973-04-25 Impact factor: 5.157

5. Molecular model for elastin structure and function.

Authors: W R Gray; L B Sandberg; J A Foster
Journal: Nature Date: 1973 Dec 21-28 Impact factor: 49.962

6. Biosynthesis of soluble elastin by pig aortic tissue in vitro.

Authors: D W Smith; W H Carnes
Journal: J Biol Chem Date: 1973-12-10 Impact factor: 5.157

7. The cross-linking of collagen and elastin: enzymatic conversion of lysine in peptide linkage to alpha-aminoadipic-delta-semialdehyde (allysine) by an extract from bone.

Authors: S R Pinnell; G R Martin
Journal: Proc Natl Acad Sci U S A Date: 1968-10 Impact factor: 11.205

Radioimmunological identification of tropoelastin.

1. Identification of a soluble intermediate during synthesis of elastin by embryonic chick aortae.

2. Biosynthesis and secretion of tropoelastin by chick aorta cells.

3. Properties of chick tropoelastin.

4. Isolation and amino acid sequences of tropoelastin peptides.

5. Molecular model for elastin structure and function.

6. Biosynthesis of soluble elastin by pig aortic tissue in vitro.

7. The cross-linking of collagen and elastin: enzymatic conversion of lysine in peptide linkage to alpha-aminoadipic-delta-semialdehyde (allysine) by an extract from bone.

8. Precipitating antibodies directed against soluble elastin - the basis of a sensitive assay.

9. Incorporation of labelled lysine into the desmosine cross-bridges in elastin.

10. Identification of a new crosslinking amino acid in elastin.

1. Immunologic localization of elastin by electron microscopy.

2. Enhancement of humoral immune response against human lung elastin peptides.