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Literature DB >> 962871

Enzymes of 2-oxo acid degradation and biosynthesis in cell-free extracts of mixed rumen micro-organisms.

Abstract

The enzymes of 2-oxo acid decarboxylation and 2-oxo acid synthesis (EC 1.2.7.1 and EC 1.2.7.2) were isolated and partially purified from cell-free extracts of rumen micro-organisms. The lyase was active with pyruvate, 3-hydroxypyruvate and 2-oxobutyrate. The synthase was active with acetate, 2-oxoglutarate or succinate. Pyruvate synthase was separated from pyruvate lyase by Sephadex G-200 gel filtration. With Sephadex filtration, approximate mol.wts. of 310000 and 210000 were determined for pyruvate lyase and pyruvate synthase respectively.

Entities: Chemical

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Year: 1976 PMID： 962871 PMCID： PMC1163857 DOI： 10.1042/bj1570325

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

19 in total

1. FERREDOXIN AS A REDUCTANT IN PYRUVATE SYNTHESIS BY A BACTERIAL EXTRACT.

Authors: R BACHOFEN; B B BUCHANAN; D I ARNON
Journal: Proc Natl Acad Sci U S A Date: 1964-04 Impact factor: 11.205

2. ROLE OF FERREDOXIN IN THE REDUCTIVE ASSIMILATION OF CO2 AND ACETATE BY EXTRACTS OF THE PHOTOSYNTHETIC BACTERIUM, CHROMATIUM.

Authors: B B BUCHANAN; R BACHOFEN; D I ARNON
Journal: Proc Natl Acad Sci U S A Date: 1964-09 Impact factor: 11.205

3. THE BIOSYNTHESIS OF ALANINE BY CLOSTRIDIUM KLUYVERI.

Authors: I G ANDREW; J G MORRIS
Journal: Biochim Biophys Acta Date: 1965-01-04

4. alpha-Ketoglutaric dehydrogenase. VII. The role of thioctic acid.

Authors: D R SANADI; M LANGLEY; F WHITE
Journal: J Biol Chem Date: 1959-01 Impact factor: 5.157

5. An electron transport factor from Clostridium pasteurianum.

Authors: L E MORTENSON; R C VALENTINE; J E CARNAHAN
Journal: Biochem Biophys Res Commun Date: 1962-06-04 Impact factor: 3.575

6. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

7. Fatty acid synthesis by enzyme preparations of Clostridium kluyveri. VI. Reactions of acyl phosphates.

Authors: E R STADTMAN; H A BARKER
Journal: J Biol Chem Date: 1950-06 Impact factor: 5.157

8. Pyruvate: ferredoxin oxidoreductase. II. Characteristics of the forward and reverse reactions and properties of the enzyme.

Authors: S Raeburn; J C Rabinowitz
Journal: Arch Biochem Biophys Date: 1971-09 Impact factor: 4.013

9. Pyruvate-ferredoxin oxidoreductase. IV. Studies on the reaction mechanism.

Authors: K Uyeda; J C Rabinowitz
Journal: J Biol Chem Date: 1971-05-25 Impact factor: 5.157

10. The synthesis of alpha-ketoglutarate from succinate and carbon dioxide by a subcellular preparation of a photosynthetic bacterium.

Authors: B B Buchanan; M C Evans
Journal: Proc Natl Acad Sci U S A Date: 1965-10 Impact factor: 11.205

5 in total

1. A reverse KREBS cycle in photosynthesis: consensus at last.

Authors: B B Buchanan; D I Arnon
Journal: Photosynth Res Date: 1990-04 Impact factor: 3.573

2. Synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in Veillonella, Selenomonas, and Bacteriodes species.

Authors: M J Allison; I M Robinson; A L Baetz
Journal: J Bacteriol Date: 1979-12 Impact factor: 3.490