Literature DB >> 9626973

Construction of a umuC'-luxAB plasmid for the detection of mutagenic DNA repair via luminescence.

T Justus1, S M Thomas.   

Abstract

This paper describes a novel system for the detection of mutagenic DNA repair in Escherichia coli. The DNA damage inducible umuC gene of Escherichia coli has been fused to the luxAB genes from Vibrio harvleyi that encode the enzyme luciferase. Mutagenicity has been assessed semi-quantitatively by the induction of bioluminescence. This system is simple, rapid and equivalent in sensitivity to other currently available test procedures. Its use in the detection of known SOS mutagens MMS, MNNG and UV is described.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9626973     DOI： 10.1016/s0027-5107(97)00215-7

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  6 in total

1.  Genetically modified Vibrio harveyi strains as potential bioindicators of mutagenic pollution of marine environments.

Authors:  A Czyz; J Jasiecki; A Bogdan; H Szpilewska; G Wegrzyn
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters.

Authors:  Anders Norman; Lars Hestbjerg Hansen; Søren J Sørensen
Journal:  Appl Environ Microbiol       Date:  2005-05       Impact factor: 4.792

3.  A computational-experimental approach to designing a polyfunctional genosensor derived from the Escherichia coli gene yfiA promoter.

Authors:  N V Tikunova; T M Khlebodarova; A V Kachko; I L Stepanenko; N A Kolchanov
Journal:  Dokl Biochem Biophys       Date:  2007 Nov-Dec       Impact factor: 0.788

4.  Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids.

Authors:  Keryn L Simons; Susan M Thomas; Peter A Anderson
Journal:  J Microbiol       Date:  2010-11-03       Impact factor: 3.422

5.  Prediction of genotoxicity of various environmental pollutants by artificial neural network simulation.

Authors:  Ryo Shoji; Masato Kawakami
Journal:  Mol Divers       Date:  2006-06-27       Impact factor: 2.943

Review 6.  Bacterial genotoxicity bioreporters.

Authors:  Alva Biran; Sharon Yagur-Kroll; Rami Pedahzur; Sebastian Buchinger; Georg Reifferscheid; Hadar Ben-Yoav; Yosi Shacham-Diamand; Shimshon Belkin
Journal:  Microb Biotechnol       Date:  2009-12-29       Impact factor: 5.813
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.