J A Nightingale1, D F Rogers, P J Barnes. 1. Thoracic Medicine, Imperial College School of Medicine, National Heart and Lung Institute, London, UK.
Abstract
BACKGROUND: Sputum induction is becoming more commonly used to assess airway inflammation. Since it is a relatively non-invasive procedure it may be useful for repeated measurements over a short period of time. METHODS: To assess the repeatability of the method over a 24 hour period, eight healthy, non-smoking, non-atopic subjects (four men) of mean age 30 years underwent sputum induction, repeated at eight hours and 24 hours. Sputum was induced by inhalation of 3.5% saline. Absolute and differential counts of inflammatory cells were performed on the whole sputum after dilution in Hank's balanced salt solution containing 1% dithiothreitol to solubilise the mucus content of the samples. RESULTS: There was a significant rise in the percentage of neutrophils in the eight hour sample compared with the baseline (57%, range 25-94% at eight hours compared with 28%, range 19-60%: median difference 26%). The median baseline percentage of macrophages was 55% (range 26-69%) which fell to 38% (range 3-56%: median difference 22%) at eight hours and 19% (range 14-59%: median difference 25%) at 24 hours. There was no significant change in the differential counts of eosinophils, lymphocytes or columnar epithelial cells, nor in any of the absolute cell counts, at any time point. CONCLUSIONS: Sputum induction may have limited utility in serial assessment of neutrophilic airway inflammation in normal subjects within a 24 hour period.
BACKGROUND: Sputum induction is becoming more commonly used to assess airway inflammation. Since it is a relatively non-invasive procedure it may be useful for repeated measurements over a short period of time. METHODS: To assess the repeatability of the method over a 24 hour period, eight healthy, non-smoking, non-atopic subjects (four men) of mean age 30 years underwent sputum induction, repeated at eight hours and 24 hours. Sputum was induced by inhalation of 3.5% saline. Absolute and differential counts of inflammatory cells were performed on the whole sputum after dilution in Hank's balanced salt solution containing 1% dithiothreitol to solubilise the mucus content of the samples. RESULTS: There was a significant rise in the percentage of neutrophils in the eight hour sample compared with the baseline (57%, range 25-94% at eight hours compared with 28%, range 19-60%: median difference 26%). The median baseline percentage of macrophages was 55% (range 26-69%) which fell to 38% (range 3-56%: median difference 22%) at eight hours and 19% (range 14-59%: median difference 25%) at 24 hours. There was no significant change in the differential counts of eosinophils, lymphocytes or columnar epithelial cells, nor in any of the absolute cell counts, at any time point. CONCLUSIONS: Sputum induction may have limited utility in serial assessment of neutrophilic airway inflammation in normal subjects within a 24 hour period.
Authors: M M Pizzichini; T A Popov; A Efthimiadis; P Hussack; S Evans; E Pizzichini; J Dolovich; F E Hargreave Journal: Am J Respir Crit Care Med Date: 1996-10 Impact factor: 21.405
Authors: P G Gibson; A Girgis-Gabardo; M M Morris; S Mattoli; J M Kay; J Dolovich; J Denburg; F E Hargreave Journal: Thorax Date: 1989-09 Impact factor: 9.139
Authors: E Bacci; S Cianchetti; P L Paggiaro; S Carnevali; L Bancalari; F L Dente; A Di Franco; D Giannini; B Vagaggini; C Giuntini Journal: Clin Exp Allergy Date: 1996-12 Impact factor: 5.018
Authors: Ashley G Henderson; Wayne H Anderson; Agathe Ceppe; Raymond D Coakley; Brian Button; Neil E Alexis; David B Peden; Eduardo R Lazarowski; C W Davis; Fred Fuller; Martha Almond; Bahjat Qaqish; Mehmet Kesimer; Richard C Boucher Journal: COPD Date: 2019-02-04 Impact factor: 2.409
Authors: Simon Y Graeber; Zhe Zhou-Suckow; Jolanthe Schatterny; Stephanie Hirtz; Richard C Boucher; Marcus A Mall Journal: Am J Respir Cell Mol Biol Date: 2013-09 Impact factor: 6.914