OBJECTIVE: To evaluate the efficacy of a commercially available bacterin-toxoid vaccine for preventing Corynebacterium pseudotuberculosis-induced abscesses in sheep. DESIGN: Prospective randomized controlled trial. ANIMALS: 31 mixed-breed sheep seronegative for C pseudotuberculosis. PROCEDURE: Sheep were randomly assigned to vaccinate (n = 20) or nonvaccinate (11; control) groups. Sheep in the vaccinate group received 2 doses of serial A or serial B bacterin-toxoid vaccine at 4-week intervals. Serologic testing was conducted after vaccination to document an antibody response to vaccination. All sheep were challenge inoculated with virulent C pseudotuberculosis organisms 32 weeks after the second vaccination. Twenty weeks after challenge inoculation, all sheep were examined for external and internal abscesses secondary to C pseudotuberculosis infection. RESULTS: Vaccinated sheep developed an antibody response to both components of the vaccine, as measured by use of ELISA tests. After challenge inoculation, vaccinated sheep had significantly less external, internal, and total abscesses than control sheep. CLINICAL IMPLICATIONS: Vaccination of sheep with a commercially available bacterin-toxoid against C pseudotuberculosis could substantially decrease the prevalence and number of abscesses that form secondary to C pseudotuberculosis infection.
OBJECTIVE: To evaluate the efficacy of a commercially available bacterin-toxoid vaccine for preventing Corynebacterium pseudotuberculosis-induced abscesses in sheep. DESIGN: Prospective randomized controlled trial. ANIMALS: 31 mixed-breed sheep seronegative for C pseudotuberculosis. PROCEDURE: Sheep were randomly assigned to vaccinate (n = 20) or nonvaccinate (11; control) groups. Sheep in the vaccinate group received 2 doses of serial A or serial B bacterin-toxoid vaccine at 4-week intervals. Serologic testing was conducted after vaccination to document an antibody response to vaccination. All sheep were challenge inoculated with virulent C pseudotuberculosis organisms 32 weeks after the second vaccination. Twenty weeks after challenge inoculation, all sheep were examined for external and internal abscesses secondary to C pseudotuberculosis infection. RESULTS: Vaccinated sheep developed an antibody response to both components of the vaccine, as measured by use of ELISA tests. After challenge inoculation, vaccinated sheep had significantly less external, internal, and total abscesses than control sheep. CLINICAL IMPLICATIONS: Vaccination of sheep with a commercially available bacterin-toxoid against C pseudotuberculosis could substantially decrease the prevalence and number of abscesses that form secondary to C pseudotuberculosis infection.
Authors: Jerônimo C Ruiz; Vívian D'Afonseca; Artur Silva; Amjad Ali; Anne C Pinto; Anderson R Santos; Aryanne A M C Rocha; Débora O Lopes; Fernanda A Dorella; Luis G C Pacheco; Marcília P Costa; Meritxell Z Turk; Núbia Seyffert; Pablo M R O Moraes; Siomar C Soares; Sintia S Almeida; Thiago L P Castro; Vinicius A C Abreu; Eva Trost; Jan Baumbach; Andreas Tauch; Maria Paula C Schneider; John McCulloch; Louise T Cerdeira; Rommel T J Ramos; Adhemar Zerlotini; Anderson Dominitini; Daniela M Resende; Elisângela M Coser; Luciana M Oliveira; André L Pedrosa; Carlos U Vieira; Cláudia T Guimarães; Daniela C Bartholomeu; Diana M Oliveira; Fabrício R Santos; Élida Mara Rabelo; Francisco P Lobo; Glória R Franco; Ana Flávia Costa; Ieso M Castro; Sílvia Regina Costa Dias; Jesus A Ferro; José Miguel Ortega; Luciano V Paiva; Luiz R Goulart; Juliana Franco Almeida; Maria Inês T Ferro; Newton P Carneiro; Paula R K Falcão; Priscila Grynberg; Santuza M R Teixeira; Sérgio Brommonschenkel; Sérgio C Oliveira; Roberto Meyer; Robert J Moore; Anderson Miyoshi; Guilherme C Oliveira; Vasco Azevedo Journal: PLoS One Date: 2011-04-18 Impact factor: 3.240
Authors: Sandip Chakraborty; Amit Kumar; Ruchi Tiwari; Anu Rahal; Yash Malik; Kuldeep Dhama; Amar Pal; Minakshi Prasad Journal: Vet Med Int Date: 2014-06-15
Authors: Syed Shah Hassan; Luis Carlos Guimarães; Ulisses de Pádua Pereira; Arshad Islam; Amjad Ali; Syeda Marriam Bakhtiar; Dayana Ribeiro; Anderson Rodrigues Dos Santos; Siomar de Castro Soares; Fernanda Dorella; Anne Cybelle Pinto; Maria Paula Cruz Schneider; Maria Silvanira Barbosa; Síntia Almeida; Vinícius Abreu; Flávia Aburjaile; Adriana Ribeiro Carneiro; Louise Teixeira Cerdeira; Karina Fiaux; Eudes Barbosa; Carlos Diniz; Flavia S Rocha; Rommel Thiago Jucá Ramos; Neha Jain; Sandeep Tiwari; Debmalya Barh; Anderson Miyoshi; Borna Müller; Artur Silva; Vasco Azevedo Journal: Stand Genomic Sci Date: 2012-12-15
Authors: Andréia Pacheco de Souza; Vera Lúcia Costa Vale; Marcos da Costa Silva; Inara Barbosa de Oliveira Araújo; Soraya Castro Trindade; Lília Ferreira de Moura-Costa; Gabriele Costa Rodrigues; Tatiane Santana Sales; Heidiane Alves dos Santos; Paulo Cirino de Carvalho-Filho; Milton Galdino de Oliveira-Neto; Robert Eduard Schaer; Roberto Meyer Journal: BMC Microbiol Date: 2014-09-02 Impact factor: 3.605