Literature DB >> 9620428

A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide.

P M Desmarchelier1, S S Bilge, N Fegan, L Mills, J C Vary, P I Tarr.   

Abstract

A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected < 1 CFU of E. coli O157 per ml in raw milk following enrichment.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9620428      PMCID： PMC104928     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus.

Authors:  S S Bilge; J C Vary; S F Dowell; P I Tarr
Journal:  Infect Immun       Date:  1996-11       Impact factor: 3.441

2.  Identification of Escherichia coli serotype O157:H7 by DNA probe specific for an allele of uid A gene.

Authors:  P Feng
Journal:  Mol Cell Probes       Date:  1993-04       Impact factor: 2.365

3.  A swimming-associated outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 and Shigella sonnei.

Authors:  W E Keene; J M McAnulty; F C Hoesly; L P Williams; K Hedberg; G L Oxman; T J Barrett; M A Pfaller; D W Fleming
Journal:  N Engl J Med       Date:  1994-09-01       Impact factor: 91.245

4.  Sequence of a variant Shiga-like toxin type-I operon of Escherichia coli O111:H-.

Authors:  A W Paton; J C Paton; P N Goldwater; M W Heuzenroeder; P A Manning
Journal:  Gene       Date:  1993-07-15       Impact factor: 3.688

5.  Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.

Authors:  V P Gannon; R K King; J Y Kim; E J Thomas
Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

Review 6.  Escherichia coli O157:H7: clinical, diagnostic, and epidemiological aspects of human infection.

Authors:  P I Tarr
Journal:  Clin Infect Dis       Date:  1995-01       Impact factor: 9.079

7.  Virulence markers of Shiga-like toxin-producing Escherichia coli strains originating from healthy domestic animals of different species.

Authors:  L Beutin; D Geier; S Zimmermann; H Karch
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

8.  Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.

Authors:  T A Cebula; W L Payne; P Feng
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948

9.  Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction.

Authors:  H Karch; T Meyer
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

10.  Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers.

Authors:  M Louie; J de Azavedo; R Clarke; A Borczyk; H Lior; M Richter; J Brunton
Journal:  Epidemiol Infect       Date:  1994-06       Impact factor: 2.451
View more
  30 in total

1.  Particular biochemical profiles for enterohemorrhagic Escherichia coli O157:H7 isolates on the ID 32E system.

Authors:  A Leclercq; B Lambert; D Pierard; J Mahillon
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

2.  Acquisition of the rfb-gnd cluster in evolution of Escherichia coli O55 and O157.

Authors:  P I Tarr; L M Schoening; Y L Yea; T R Ward; S Jelacic; T S Whittam
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

3.  Shiga Toxin-Producing Escherichia coli.

Authors: 
Journal:  Curr Infect Dis Rep       Date:  2000-02       Impact factor: 3.725

4.  Selective and sensitive method for PCR amplification of Escherichia coli 16S rRNA genes in soil.

Authors:  G Sabat; P Rose; W J Hickey; J M Harkin
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

5.  Seropathotypes, Phylogroups, Stx subtypes, and intimin types of wildlife-carried, shiga toxin-producing escherichia coli strains with the same characteristics as human-pathogenic isolates.

Authors:  Azucena Mora; Cecilia López; Ghizlane Dhabi; Ana M López-Beceiro; Luís E Fidalgo; Eduardo A Díaz; Carlos Martínez-Carrasco; Rosalía Mamani; Alexandra Herrera; Jesús E Blanco; Miguel Blanco; Jorge Blanco
Journal:  Appl Environ Microbiol       Date:  2012-02-03       Impact factor: 4.792

6.  Escherichia coli O157 somatic antigen is present in an isolate of E. fergusonii.

Authors:  Narelle Fegan; Robert Steven Barlow; Kari Steven Gobius
Journal:  Curr Microbiol       Date:  2006-05-09       Impact factor: 2.188

7.  Variability of Escherichia coli O157 strain survival in manure-amended soil in relation to strain origin, virulence profile, and carbon nutrition profile.

Authors:  Eelco Franz; Angela H A M van Hoek; El Bouw; Henk J M Aarts
Journal:  Appl Environ Microbiol       Date:  2011-09-09       Impact factor: 4.792

8.  Analysis of yeh Fimbrial Gene Cluster in Escherichia coli O157:H7 in Order to Find a Genetic Marker for this Serotype.

Authors:  Hadi Ravan; Mojdeh Amandadi
Journal:  Curr Microbiol       Date:  2015-06-03       Impact factor: 2.188

9.  Epidemiological survey on Escherichia coli O157 in Chongqing and Three-Gorge Reservoir Areas of China.

Authors:  Haoju Wang; Xuhu Mao; Honglei Ding; Quanming Zou; Xiao Peng
Journal:  Vet Res Commun       Date:  2008-05-15       Impact factor: 2.459

10.  Nissui glucose fermentative gram-negative rod identification system EB-20 gives a unique profile for typical non-sorbitol-fermenting Escherichia coli O157:H7.

Authors:  H Kodaka; Y Uesaka; F Kashitani
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.