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Literature DB >> 9620427

Stabilized, freeze-dried PCR mix for detection of mycobacteria.

P R Klatser¹, S Kuijper, C W van Ingen, A H Kolk.

Abstract

We report here the development of a freeze-drying procedure allowing stabilization at ambient temperature of preoptimized, premixed, and predispensed PCR mixes aimed at the detection of mycobacteria in clinical materials. The freeze-dried mixes retained activity at 4 degrees C and at 20 degrees C for 1 year and for 3 months at 37 degrees C, as judged by their performance with 50 and 500 fg of purified Mycobacterium bovis BCG target DNA.

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Year: 1998 PMID： 9620427 PMCID： PMC104927

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

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