Literature DB >> 9620425

Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors.

J A Higgins1, S Radulovic, B H Noden, J M Troyer, A F Azad.   

Abstract

We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9620425      PMCID： PMC104925     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Genetic characterization and transovarial transmission of a typhus-like rickettsia found in cat fleas.

Authors:  A F Azad; J B Sacci; W M Nelson; G A Dasch; E T Schmidtmann; M Carl
Journal:  Proc Natl Acad Sci U S A       Date:  1992-01-01       Impact factor: 11.205

2.  Transcriptional regulation in the obligate intracytoplasmic bacterium Rickettsia prowazekii.

Authors:  J Cai; H H Winkler
Journal:  J Bacteriol       Date:  1996-09       Impact factor: 3.490

3.  A comparison of three methods for isolating RNA from mosquitoes.

Authors:  F G Noriega; M A Wells
Journal:  Insect Mol Biol       Date:  1993       Impact factor: 3.585

4.  Transcriptional analysis of the 16s rRNA gene in Rickettsia prowazekii.

Authors:  H Pang; H H Winkler
Journal:  J Bacteriol       Date:  1996-03       Impact factor: 3.490

5.  Cloning and sequence analysis of the gene encoding the crystalline surface layer protein of Rickettsia typhi.

Authors:  M J Hahn; K K Kim; I Kim; W H Chang
Journal:  Gene       Date:  1993-10-29       Impact factor: 3.688

6.  The large difference in infectivity for mice of Plasmodium berghei and Plasmodium yoelii sporozoites cannot be correlated with their ability to enter into hepatocytes.

Authors:  M R Briones; M Tsuji; V Nussenzweig
Journal:  Mol Biochem Parasitol       Date:  1996-04       Impact factor: 1.759

7.  Identification of tlc and gltA mRNAs and determination of in situ RNA half-life in Rickettsia prowazekii.

Authors:  J Cai; H H Winkler
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

8.  Identification and characterization of epitopes on the 120-kilodalton surface protein antigen of Rickettsia prowazekii with synthetic peptides.

Authors:  W M Ching; H Wang; B Jan; G A Dasch
Journal:  Infect Immun       Date:  1996-04       Impact factor: 3.441

9.  The concentrations of stable RNA and ribosomes in Rickettsia prowazekii.

Authors:  H Pang; H H Winkler
Journal:  Mol Microbiol       Date:  1994-04       Impact factor: 3.501
  9 in total
  3 in total

1.  Functional characterization of a phospholipase A(2) homolog from Rickettsia typhi.

Authors:  M Sayeedur Rahman; Nicole C Ammerman; Khandra T Sears; Shane M Ceraul; Abdu F Azad
Journal:  J Bacteriol       Date:  2010-04-30       Impact factor: 3.490

2.  Transcriptional analysis of Rickettsia prowazekii invasion gene homolog (invA) during host cell infection.

Authors:  Jariyanart Gaywee; Suzana Radulovic; James A Higgins; Abdu F Azad
Journal:  Infect Immun       Date:  2002-11       Impact factor: 3.441

3.  Rickettsia-macrophage interactions: host cell responses to Rickettsia akari and Rickettsia typhi.

Authors:  S Radulovic; P W Price; M S Beier; J Gaywee; J A Macaluso; A Azad
Journal:  Infect Immun       Date:  2002-05       Impact factor: 3.441
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.