Literature DB >> 96199

The picomole determination of free and total cholesterol in cells in culture.

J G Heider, R L Boyett.   

Abstract

An enzymatic, fluorometric method for the determination of free and total cholesterol in cells in culture is presented. The method is simple, requiring one reagent that includes all of the enzymes and a second reagent that increases the pH, which enhances the fluorescence of the product. The method is based on the enzymatic hydrolysis of cholesteryl esters to free cholesterol, the oxidation of cholesterol with the liberation of hydrogen peroxide, and the reaction of this peroxide with a fluorogen to form a fluoresencet product in the presence of a peroxidase. It is rapid, in that free cholesterol can be read in 5 minutes and total cholesterol after 20 minutes. The precision of the method is greater than that obtained from gas-liquid chromatography.

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Year:  1978        PMID： 96199

Source DB:  PubMed          Journal:  J Lipid Res        ISSN： 0022-2275            Impact factor:   5.922


  77 in total

1.  Relationship of lipid rafts to transient confinement zones detected by single particle tracking.

Authors:  Christian Dietrich; Bing Yang; Takahiro Fujiwara; Akihiro Kusumi; Ken Jacobson
Journal:  Biophys J       Date:  2002-01       Impact factor: 4.033

2.  Asymmetric requirement for cholesterol in receptor-bearing but not envelope-bearing membranes for fusion mediated by ecotropic murine leukemia virus.

Authors:  Xiongbin Lu; Ying Xiong; Jonathan Silver
Journal:  J Virol       Date:  2002-07       Impact factor: 5.103

3.  A simple and sensitive enzymatic method for cholesterol quantification in macrophages and foam cells.

Authors:  Peggy Robinet; Zeneng Wang; Stanley L Hazen; Jonathan D Smith
Journal:  J Lipid Res       Date:  2010-08-04       Impact factor: 5.922

4.  High glucose levels do not directly impair cellular binding of HDL3 or HDL-mediated efflux of cholesterol from human skin fibroblasts.

Authors:  P B Duell; E L Bierman
Journal:  Acta Diabetol       Date:  1991       Impact factor: 4.280

5.  Analyses of group III secreted phospholipase A2 transgenic mice reveal potential participation of this enzyme in plasma lipoprotein modification, macrophage foam cell formation, and atherosclerosis.

Authors:  Hiroyasu Sato; Rina Kato; Yuki Isogai; Go-ichi Saka; Mitsuhiro Ohtsuki; Yoshitaka Taketomi; Kei Yamamoto; Kae Tsutsumi; Joe Yamada; Seiko Masuda; Yukio Ishikawa; Toshiharu Ishii; Tetsuyuki Kobayashi; Kazutaka Ikeda; Ryo Taguchi; Shinji Hatakeyama; Shuntaro Hara; Ichiro Kudo; Hiroyuki Itabe; Makoto Murakami
Journal:  J Biol Chem       Date:  2008-09-18       Impact factor: 5.157

6.  The effect of low density lipoprotein composition on the regulation of cellular cholesterol synthesis: a comparison in diabetic and non-diabetic subjects.

Authors:  D Owens; S McBrinn; P Collins; A Johnson; G H Tomkin
Journal:  Acta Diabetol       Date:  1993       Impact factor: 4.280

7.  Human noninsulin-dependent diabetes: identification of a defect in plasma cholesterol transport normalized in vivo by insulin and in vitro by selective immunoadsorption of apolipoprotein E.

Authors:  C J Fielding; G M Reaven; P E Fielding
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

8.  Identification of core aldehydes among in vitro peroxidation products of cholesteryl esters.

Authors:  H Kamido; A Kuksis; L Marai; J J Myher
Journal:  Lipids       Date:  1993-04       Impact factor: 1.880

9.  Ezetimibe inhibits expression of acid sphingomyelinase in liver and intestine.

Authors:  Yajun Cheng; Fuli Liu; Jun Wu; Yao Zhang; Ake Nilsson; Rui-Dong Duan
Journal:  Lipids       Date:  2009-09-24       Impact factor: 1.880

10.  Characterization of hepatic low density lipoprotein binding and cholesterol metabolism in normal and homozygous familial hypercholesterolemic subjects.

Authors:  J M Hoeg; S J Demosky; E J Schaefer; T E Starzl; H B Brewer
Journal:  J Clin Invest       Date:  1984-02       Impact factor: 14.808
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