| Literature DB >> 9592176 |
T C Koekemoer1, T G Downing, W Oelofsen.
Abstract
A method is described for the quantification of mitochondrial DNA present in crude biological preparations. A known copy number of a standard is amplified in the presence of inactivated target DNA so as to determine the overall efficiency of the PCR process in a particular sample. In this way any inhibitory and/or stimulatory substances present in sample preparations can be taken into account. To reduce tube-to-tube variations product DNA quantification is limited to small cycle numbers. Using this method quantitations of DNA amounts in different crude preparations can be compared.Mesh：
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Year: 1998 PMID： 9592176 PMCID： PMC147619 DOI： 10.1093/nar/26.11.2829
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971